HPLC法测定胃镜消泡剂中羟苯乙酯钠含量  

Determination of Content of Sodium Ethylparaben in Gastroscope Defoamer by HPLC

作　　者：殷敏 胡尊汉 李秋璐 孙颖 董自波 付免 YIN Min;HU Zun-han;LI Qiu-lu;SUN Ying;DONG Zi-bo;FU Mian(Pharmacy School,Jiangsu Ocean University,Lianyungang Jiangsu 222000,China)

机构地区：[1]江苏海洋大学药学院,江苏连云港222000

出　　处：《当代化工》2024年第1期184-187,199,共5页Contemporary Chemical Industry

基　　金：江苏省高等学校自然科学面上项目,含非蛋白质氨基酸的KLA类似物抗肿瘤活性及机制研究(项目编号:21KJB350019);江苏省海洋药物活性分子筛选重点实验室开放课题,新型诱导肿瘤凋亡肽的设计、合成与活性评估(项目编号:HY202003);江苏海洋大学人才引进科研基金项目,博士科研启动费。

摘　　要：采用高效液相色谱(HPLC)法测定胃镜消泡剂中羟苯乙酯钠的含量,色谱条件:采用Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-1%冰乙酸溶液(60∶40)为流动相,流速为1.0 mL·min^(-1),检测波长为256 nm,柱温为30℃。结果表明:羟苯乙酯钠在2.41~24.14μg·mL^(-1)范围内线性关系良好(r=1.000 0),平均回收率为107.02%,RSD为1.37%。该方法简便、准确,可用于胃镜消泡剂中羟苯乙酯钠的含量控制。Sodium ethylparaben content in gastroscope defoamer was detected by using HPLC,with the column of Kromasil C_(18) (250 mm×4.6 mm,5μm).The mobile phase was methano-1%acetic acid solution (60∶40) at a flow rate was 1.0 mL·min^(-1).The detection wavelength was set at 256 nm.The column temperature was 30℃.The results showed that sodium ethylparaben had a good linear relationship in the range of 2.41~24.14μg·mL^(-1) (r=1.000 0).The average recovery was 107.02%with RSD of 1.37%.The established method was simple and accurate,and could be used for the quality control of sodium ethylparaben agent in gastroscope defoamer.

关 键 词：高效液相色谱 胃镜消泡剂 羟苯乙酯钠 含量 方法学验证 

分 类 号：TQ460.72[医药卫生—药物分析学]