土壤中辛硫磷和高效氯氟氰菊酯残留量的高效液相色谱分析  

Analysis of Phoxim and Lambda-cyhalothrin in Soil by High Performance Liquid Chromatography

作　　者：刘博静 王立霞 孙阳阳 张翠华 程梦圆 LIU Bo-jing;WANG Li-xia;SUN Yang-yang;ZHANG Cui-hua;CHENG Meng-yuan(College of Chemistry and Chemical Engineering,Cangzhou Normal University,Cangzhou,Hebei 061001,China)

机构地区：[1]沧州师范学院化学与化工学院,河北沧州061001

出　　处：《沧州师范学院学报》2024年第1期6-9,共4页Journal of Cangzhou Normal University

基　　金：2021年度沧州市重点研发计划指导项目“超声萃取高效液相色谱法检测土壤中辛硫磷和高效氯氟氰菊酯的残留量”,编号:No.213203001。

摘　　要：从土壤中超声提取辛硫磷和高效氯氟氰菊酯,并采用高效液相色谱法检测其残留量.用乙腈-水(体积比4∶1)作为提取剂,对土壤样品进行超声萃取,提取液经弗罗里硅土小柱净化后,采用反相高效液相色谱法进行残留量测定.使用SynergiTMPolar-RP 80?(250 mm×4.6 mm, 4μm)色谱柱,以乙腈-水(体积比80∶20)为流动相,紫外检测波长为225 nm.结果表明:辛硫磷与高效氯氟氰菊酯的萃取率均高于92%.辛硫磷浓度在0.6~5.4μg/mL范围内和峰面积线性关系良好,线性回归方程A=25.108ρ+45.008,r=0.998 9;高效氯氟氰菊酯浓度在0.4~3.6μg/mL范围内与峰面积呈良好线性关系,线性回归方程A=48.835ρ+56.574,r=0.999 0.该方法简单、快速、准确度高、精密度好,能满足土壤中辛硫磷和高效氯氟氰菊酯残留量的检测要求.A method was developed for the ultrasonic extraction of phoxim and lambda-cyhalothrin from soil and the detection of their residues by high performance liquid chromatography.The soil samples were ultrasonic extraction by acetonitrile-water(4∶1,by vol)as extractant.The extract was purified with Floridian column.The residue was determined by reversed phase high performance liquid chromatography.With Synergi TM Polar-RP 80(250 mm×4.6 mm,4μm)column was used as the fix phase,the mixture of acetonitrile and water(80∶20,by vol)was used as the mobile phase,the UV detector wavelength was 225 nm.Results show that both phoxim and lambda-cyhalothrin exhibit extraction rates exceeding 92%.The concentration and peak area of phoxim exhibit a good linear relationship between 0.6~5.4μg/mL,and the linear regression equation is A=25.108ρ+45.008,r=0.9989.The concentration and peak area of high-efficiency cypermethrin exhibit a good linear relationship between 0.4~3.6μg/mL,and the linear regression equation is A=48.835ρ+56.574,r=0.9990.The method is simple,rapid,of high accuracy and precision,and can meet the requirements for detecting phoxim and lambda-cyhalothrin residues in soil.

关 键 词：高效液相色谱 辛硫磷 高效氯氟氰菊酯 土壤 残留 

分 类 号：O657.7[理学—分析化学]