白术多糖对Cr(Ⅵ)诱导PK-15细胞凋亡和小鼠肾损伤的影响  

Effects of Polysaccharide from Atractylodes macrocephala Koidz.on Cr(Ⅵ)-Induced Apoptosis in PK-15 Cells and Renal Injury in Mice

作　　者：吕昌洋 范汝鹏 赵茜曼 高忆凡 蒋猛林 李林珏 郑丕苗 刘建柱[1] 赵晓娜[1] Lü Chang-yang;FAN Ru-peng;ZHAO Xi-man;GAO Yi-fan;JIANG Meng-lin;LI Lin-jue;ZHENG Pi-miao;LIU Jian-zhu;ZHAO Xiao-na(College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai′an,Shandong,271018,China)

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《动物医学进展》2024年第3期59-66,共8页Progress In Veterinary Medicine

基　　金：山东省自然科学基金项目(ZR2021MC088)。

摘　　要：为探讨白术多糖(RAMPS_(t))对Cr(Ⅵ)致PK-15细胞凋亡和小鼠肾损伤的保护作用,为临床中药多糖缓解重金属中毒导致肾损伤提供理论依据。采用RAMPS_(t)干预Cr(Ⅵ)所致PK-15细胞损伤,检测细胞存活率、ROS水平、线粒体膜电位、细胞凋亡率及Bax、Bcl-2蛋白表达水平,测定MDA、SOD和GSH含量。为了进一步验证RAMPS_(t)对Cr(Ⅵ)致小鼠肾氧化应激及凋亡的保护作用,检测肾组织中ROS、MDA、SOD、GSH的变化及Bcl-2、Bax的表达水平。结果显示,RAMPS_(t)缓解Cr(Ⅵ)引起细胞内ROS水平升高,线粒体膜电位下降,MDA水平升高及SOD、GSH水平降低,且减少细胞凋亡率。同时,RAMPS_(t)增强Bcl-2蛋白表达水平,降低Bax蛋白表达水平。RAMPS_(t)干预可抑制Cr(Ⅵ)致小鼠ROS、MDA水平升高和SOD、GSH水平下降;并且降低Bax蛋白表达水平,增加Bcl-2蛋白的表达水平,减轻Cr(Ⅵ)诱导的小鼠肾脏细胞凋亡。因此,RAMPS_(t)通过降低ROS水平,阻断氧化应激介导的线粒体凋亡途径,从而拮抗Cr(Ⅵ)致PK-15细胞和小鼠肾氧化应激及凋亡的作用。The aim was to explore the protective effect of Atractylodes macrocephala Koidz.(RAMPS_(t))on Cr(Ⅵ)-induced apoptosis of PK-15 cells and renal injury in mice,and to provide a theoretical basis for clinical Chinese medicine polysaccharides to alleviate renal injury caused by heavy metal poisoning.Firstly,RAMPS_(t) was used to intervene Cr(Ⅵ)-induced PK-15 cell injury.Cell viability,ROS level,mitochondrial membrane potential and apoptosis rate were detected.The contents of MDA,SOD and GSH were measured.The expression levels of Bax,Bcl-2 proteins were detected.In order to further verify the protective effect of RAMPS_(t) on Cr(Ⅵ)-induced renal oxidative stress and apoptosis in mice,the changes of ROS,MDA,SOD,GSH and the expression levels of Bcl-2 and Bax in renal tissue were detected.The results showed that RAMPS_(t) alleviated the increase of intracellular ROS level,the decrease of mitochondrial membrane potential,the increase of MDA level and the decrease of SOD and GSH levels caused by Cr(Ⅵ),and reduced the apoptosis rate.Meanwhile,RAMPS_(t) enhanced Bcl-2 protein expression level and decreased the expression levels of Bax protein expression.RAMPS_(t) intervention inhibited the increased levels of ROS and MDA and the decreased levels of SOD and GSH in mice induced by Cr(Ⅵ).RAMPS_(t) decreased the expression level of Bax,increased the expression level of Bcl-2,and eventually induced the apoptosis of mice kidney cells.Therefore,RAMPS_(t) antagonizes Cr(Ⅵ)-induced oxidative stress and apoptosis in PK-15 cells and mouse kidneys by reducing ROS levels and blocking oxidative stress-mediated mitochondrial apoptosis pathway.

关 键 词：白术多糖 Cr(Ⅵ) 氧化应激 细胞凋亡 PK-15细胞 

分 类 号：S853.7[农业科学—临床兽医学]