马麝出血症组织灭活疫苗的制备及免疫保护性分析  

Preparation of Tissue Inactivated Vaccine against Hemorrhagic Disease of Musk Deer and Analysis of Its Immune Protection

作　　者：马海云 张玉清 郭潞莎 包世俊[1] MA Hai-yun;ZHANG Yu-qing;GUO Lu-sha;BAO Shi-jun(College of Veterinary Medicine,Gansu Agriculture University,Lanzhou,Gansu,730070,China)

机构地区：[1]甘肃农业大学动物医学学院,甘肃兰州730070

出　　处：《动物医学进展》2024年第3期78-81,共4页Progress In Veterinary Medicine

基　　金：甘肃省重点研发计划项目(20YF3NA015);甘肃农业大学省级大学生创新创业训练计划(202010733009)。

摘　　要：马麝病毒性出血症(Moschus chrysogaster viral hemorrhagic disease,McVHD)是一种急性、高度致死性、出血性传染病,给马麝驯养业造成了重大的经济损失,严重威胁马麝种群的繁育以及相关产业的发展。为了提高对马麝出血症的防控能力,需研制有效预防马麝出血症的疫苗。用出血症病死马麝的肝脏毒感染家兔,取病死兔肝脏制备组织匀浆,反复冻融后离心,上清经除菌处理后用甲醛灭活,进而配制马麝出血症组织灭活疫苗,并在安全性试验的基础上进行动物试验。结果显示,未加佐剂组织灭活疫苗组的致死保护率为100%,白油佐剂组织灭活疫苗组的致死保护率为66.7%,商品疫苗组致死保护率为100%,表明疫苗有较好免疫保护效果。研究结果为有效预防马麝出血症和商品化疫苗的开发奠定了基础。The hemorrhagic disease of musk deer is an acute,highly lethal hemorrhagic disease prevalent in the musk deer population,which has caused great economic losses to the domestication industry of Chinese musk deer and seriously threatened the breeding of musk deer population and the development of related industries.In order to improve the ability of prevention and control of the disease in musk deers,it is necessary to develop effective vaccines against musk deer haemorrhage.In this study,the rabbits were infected with the liver virus from dead musk deer,and tissue homogenates were prepared from the liver of dead musk deer,which were repeatedly centrifuged after freezing and thawing.The supernatant was desterilized and then inactivated with formaldehyde,and then the tissue inactivated vaccine was prepared.On the basis of safety tests,the protective effect of the vaccine was analyzed through animal tests.The results showed that the lethal protection rate of the inactivated vaccine group without adjuvant was 100%,that of the inactivated vaccine group with white oil adjuvant was 66.7%,and that of the commercial vaccine group was 100%,indicating that the vaccine had a good immune protection effect.The results of this study laid the foundation and provided the experimental basis for the effective prevention of hemorrhagic disease of musk deer,and the development of commercial vaccine.

关 键 词：马麝出血症 灭活疫苗 白油佐剂 

分 类 号：S852.4[农业科学—基础兽医学]