向川安瘿蜂寄生白栎虫瘿和成瘿枝的代谢组比较  

Comparison of Metabolome of Quercus fabri Twigs and Associated Galls Induced by Andricus mukaigawae

作　　者：王玉枚 杨雪梅 惠羽 朱道弘[2] 曾杨[2] 杨筱慧[1] WANG Yumei;YANG Xuemei;HUI Yu;ZHU Daohong;ZENG Yang;YANG Xiaohui(College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081 [2]中南林业科技大学生命科学与技术学院,中国湖南长沙410004

出　　处：《生命科学研究》2024年第1期48-55,共8页Life Science Research

基　　金：国家重点研发计划项目(2018YFE0127100)。

摘　　要：虫瘿是植物受致瘿昆虫产卵和取食等刺激而异常生长形成的不正常组织。营养假说认为虫瘿组织营养物质的含量高于寄主植物的成瘿部位,而次生代谢产物的含量低于寄主植物的成瘿部位。向川安瘿蜂(Andricus mukaigawae)属膜翅目(Hymenoptera)瘿蜂科(Cynipidae),在白栎(Quercus fabri)枝条上形成虫瘿。本文基于非靶向代谢组,使用高效液相色谱-质谱检测、鉴定和比较向川安瘿蜂幼虫期虫瘿与寄主植物成瘿枝的代谢物。研究结果表明,向川安瘿蜂虫瘿和成瘿枝的代谢物组成存在差异;虫瘿的脂肪含量均高于成瘿枝,虫瘿的4种单宁和4种黄酮物质的含量均低于成瘿枝,这支持营养假说;虫瘿的氨基酸含量均低于成瘿枝,部分酚类物质的含量高于成瘿枝,这不支持营养假说。Galls are the abnormal tissues formed by the abnormal growth of plants stimulated by the oviposition and feeding of gall insects.The nutritional hypothesis suggests that the content of nutrients in gall tissues is higher than that in the gall forming parts of the host plants,while the content of secondary metabolites is lower than that in the gall forming parts of host plants.Andricus mukaigawae belongs to the family Cynipidae of the order Hymenoptera.It forms galls on the branches of white oak(Quercus fabri).Herein,based on the non-targeted metabolome,metabolites in galls and host plants at the larval stage of A.mukaigawae were detected,identified and compared using high-performance liquid chromatography-mass spectrometry(HPLC-MS).The results showed that there were differences in metabolite composition between the galls and the galled branches.Lipid content of the galls was higher than that of the galled branches,and the contents of four kinds of tannins and four kinds of flavonoids of the galls were lower than those of the galled branches,which supported the nutrient hypothesis.However,the amino acid content of the galls was lower than that of the galled branches,and the content of some phenolics was higher than that of the galled branches,which did not support the nutrient hypothesis.

关 键 词：向川安瘿蜂 虫瘿 非靶向代谢组 营养假说 

分 类 号：Q948[生物学—植物学]