Identification and functional characterization of female antennae-biased odorant receptor 23 involved in acetophenone detection of the Indian meal moth Plodia interpunctella  

作　　者：Qi Chen Xiaoyan Zhu Guoqing Kang Qiling Yu Qingxin Liu Lin Du Yi Yang Xinyu He Ying Zhao Junjie Zhang Ying Hu Bingzhong Ren 

机构地区：[1]Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization,School of Life Sciences,Northeast Normal University,Changchun,China [2]Key Laboratory of Vegetation Ecology,Ministry of Education,Northeast Normal University,Changchun,China [3]Jilin Provincial Engineering Laboratory of Avian Ecology and Conservation Genetics,Northeast Normal University,Changchun,China [4]Engineering Research Center of Natural Enemies,Institute of Biological Control,Jilin Agricultural University,Changchun,China

出　　处：《Insect Science》2024年第1期59-78,共20页昆虫科学（英文版）

基　　金：This research was supported by the China Postdoctoral Science Foundation(2021M690588);the Fundamental Research Funds for the Central Universities(135111010);the Young Science and Technology Talent Support Project of Jilin Province(QT202121);The authors would like to thank all the reviewers who participated in the review,as well as MJEditor(www.mjeditor.com)for providing English editing services during the preparation of this manuscript.

摘　　要：The Indian meal moth,Plodia interpunctella(Lepidoptera:Pyralidae),a globally distributed storage pest,relies on odors that are emitted from stored foods to select a suitable substrate for oviposition.However,the molecular mechanism underlying the chemical communication between P.interpunctella and its host remains elusive.In this study,130 chemosensory genes were identified from the transcriptomes of 7 P.interpunctella tissues,and the quantitative expression levels of all 56 P.interpunctella odorant receptor genes(PintORs)were validated using real-time quantitative polymerase chain reaction.The functional characteristics of 5 PintORs with female antennae-biased expression were investigated using 2-electrode voltage clamp recordings in Xenopus laevis oocytes.PintOR23 was found to be specifically tuned to acetophenone.Acetophenone could elicit a significant electrophysiological response and only attracted mated females when compared with males and virgin females.In addition,molecular docking predicted that the hydrogen bonding sites,TRP-335 and ALA-167,might play key roles in the binding of PintOR23 to acetophenone.Our study provides valuable insights into the olfactory mechanism of oviposition substrate detection and localization in P.interpunctella and points toward the possibility of developing eco-friendly odorant agents to control pests of stored products.

关 键 词：ACETOPHENONE DOCKING EAG odorant receptor Plodia interpunctella TEVC 

分 类 号：Q96[生物学—昆虫学]