Hexamerin and allergen are required for female reproduction in the American cockroach,Periplaneta americana  

作　　者：Shiming Zhu Xiaoyi Chen Sishi Xia Qin Li Ziqi Ye Shaoting Zhao Kexin Liu Fangfang Liu 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,Institute of Insect Science and Technology&School of Life Sciences,South China Normal University,Guangzhou,China [2]Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,Guangdong Province,China [3]Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou,China

出　　处：《Insect Science》2024年第1期186-200,共15页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.32100379);the Natural Science Foundation of Guangdong Province(Grant No.2023A1515010512);the GuangDong Basic and Applied Basic Research Foundation(Grant No.2020A1515110285);the China Postdoctoral Science Foundation(Grant No.2020M672678).English was polished by the Nature Publishing Group.

摘　　要：Reproduction is of great importance for the continuation of the species.In insects,the fat body is the major tissue for nutrient storage and involved in vitellogenesis,which is essential for female reproduction.Here,2 proteins,hexamerin and allergen,were separated from the fat bodies of adult female American cockroaches(Periplaneta americana)and identified as storage proteins,encoding for 733 amino acids with molecular weight of 87.88 kDa and 686 amino acids with molecular weight of 82.18 kDa,respectively.The encoding genes of these 2 storage proteins are mainly expressed in the fat body.RNA interference-mediated knockdown of Hexamerin and Allergen in the early stage of the first reproductive cycle in females suppressed vitellogenesis and ovarian maturation,indicating that these storage proteins are involved in controlling reproduction.Importantly,the expression of Hexamerin and Allergen was repressed by knockdown of the juvenile hormone(JH)receptor gene Met and the primary response gene Kr-h1,and was induced by methoprene,a JH analog,in both in vivo and in vitro experiments.Altogether,we have determined that hexamerin and allergen are identified as storage proteins and play an important role in promoting female reproduction in the American cockroach.The expression of their encoding genes is induced by JH signaling.Our data reveal a novel mechanism by which hexamerin and allergen are necessary for JH-stimulated female reproduction.

关 键 词：fat body juvenile hormone OVARY REPRODUCTION storage protein VITELLOGENESIS 

分 类 号：Q96[生物学—昆虫学]