探究电针廉泉穴对脑卒中后吞咽困难大鼠神经功能缺损的影响  

Effect of electroacupuncture at Lianquan point on nerve function deficit in rats after stroke

作　　者：金海涛[1] 张雯[1] 王非[1] Jin Haitao;Zhang Wen;Wang Fei(Department of Encephalopathy,Wuhan Traditional Chinese Medicine Hospital,Wuhan 430014,Hubei Province,China)

机构地区：[1]武汉市中医医院脑病科,430014

出　　处：《中华老年心脑血管病杂志》2024年第1期87-91,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：武汉市医学科研项目(WZ20C36);武汉市中医药科研项目(WZ22C65)。

摘　　要：目的探讨电针廉泉穴对脑卒中后吞咽困难(PSD)大鼠神经功能缺损的影响及潜在对瞬时受体电位香草酸亚型1(TRPV1)信号通路的调节机制作用。方法选用SPF级SD雄性大鼠60只,随机分为正常组12只(仅浅插栓线,未导致脑内动脉闭塞),余48只制作PSD模型,将造模成功的36只大鼠随机分为模型组、治疗组和治疗+咖啡酸组,每组12只。记录大鼠吞咽潜伏期和吞咽次数,生物信号采集器检测舌下神经放电、舌肌阈强度和收缩幅度,酶联免疫吸附测定血清P物质含量,甲苯胺蓝染色检测舌下神经核尼氏体数目,免疫组织化学检测舌下神经核TRPV1、五羟色胺(5-HT)、磷酸化p38、神经元型一氧化氮合酶(nNOS)蛋白表达水平。结果与正常组比较,模型组大鼠吞咽潜伏期、吞咽次数、舌下神经放电积分面积、舌肌收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平下降,舌肌阈强度和舌下神经核磷酸化p38、nNOS蛋白表达水平增加(P<0.05);与模型组比较,治疗组大鼠舌肌单收缩幅度、舌肌强直收缩幅度、血清P物质含量、舌下神经核尼氏体数目、TRPV1及5-HT蛋白表达水平增加[2.36±0.26 vs 1.77±0.22、3.46±0.36 vs 2.15±0.18、(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml、(33.60±3.65)个vs(24.60±2.34)个、(19.85±2.11)%vs(9.79±1.07)%、(22.43±2.34)%vs(10.85±1.13)%,P<0.05]。结论电针廉泉穴可能通过激活TRPV1信号通路改善PSD大鼠神经功能缺损。Objective To investigate the effect of electroacupuncture at Lianquan point on nerve function deficit in post-stroke dysphagia(PSD)rats and its potential effect on regulating of transient receptor potential vanilic acid subtype 1(TRPV1)signaling pathway.Methods A total of 60 male SD rats with SPF grade were randomly divided into a normal group of 12(only mild insertion of the thread,which did not lead to intracerebral artery occlusion),and the remaining 48 rats were established into PSD models.The 36 rats successfully made were randomly divided into model group,treatment group and treatment+caffeic acid group,with 12 rats in each group.The latency and frequency of swallowing were recorded.Biological signal collector was applied to detect hypoglossal nerve discharge,lingual muscle threshold intensity,and contraction amplitude.ELISA was employed to detect the content of substance P in serum,toluidine blue staining was conducted to count the number of Nissl bodies in the hypoglossal nucleus,and immunohistochemistry was applied to measure the expression levels of TRPV1,serotonin(5-HT),phosphorylation(p)-p38,and neuronal nitric oxide synthase(nNOS)in the hypoglossal nucleus.Results Compared with the normal group,the model group had shorter swallowing latency,less swallowing frequency,and decreased integrated area of hypoglossal nerve discharge,amplitude of tongue muscle contraction,serum substance P content,threshold strength of tongue muscle,number of Nissl bodies,and the expression levels of TRPV1 and 5-HT,but increased threshold intensity of tongue muscles and expression levels of p-p38 and nNOS in the hypoglossal nucleus(P<0.05).Compared with the model group,the amplitude of tongue muscle contraction,serum substance P content,number of Nissl bodies,and the expression levels of TRPV1 and 5-HT proteins in rats in the treatment group were increased[2.36±0.26 vs 1.77±0.22,3.46±0.36 vs 2.15±0.18,(3.92±0.38)ng/ml vs(1.69±0.17)ng/ml,(33.60±3.65)vs(24.60±2.34),(19.85±2.11)%vs(9.79±1.07)%,(22.43±2.34)%vs(10.85±

关 键 词：卒中 大鼠 Sprague-Dawley 模型 动物 吞咽障碍 穴 廉泉 电针 香草酸亚型1信号通路 

分 类 号：R245.97[医药卫生—针灸推拿学]