限时饮食对高脂饮食诱导肥胖大鼠髌下脂肪垫的影响及作用机制  

Effect of time-restricted diet on infrapatellar fat pad in high-fat diet-induced obese rats and relevant mechanisms

作　　者：丁煜堃 祝翠玲 张晓东 Ding Yukun;Zhu Cuiling;Zhang Xiaodong(Department of Medical Imaging,The Third Affiliated Hospital of Southern Medical University,Guangzhou 510630,Guangdong Province,China)

机构地区：[1]南方医科大学第三附属医院影像科,广东省广州市510630

出　　处：《中国组织工程研究》2024年第34期5425-5431,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81801653),项目负责人:张晓东;南方医科大学第三附属医院院长基金项目(YM2021012),项目负责人:张晓东。

摘　　要：背景:超重或肥胖是膝骨关节炎最主要的危险因素。限时饮食在预防超重或肥胖方面显示出明显的效果。髌下脂肪垫是膝关节的重要组成部分,其是否受到限时饮食的影响,从而影响膝骨关节炎目前仍不清楚。目的:利用高脂饮食诱导的大鼠模型,探究限时饮食减重对髌下脂肪垫的影响,为肥胖相关性膝骨关节炎的早期预防和治疗提供证据。方法:将15只雄性SD大鼠随机分为3组,每组5只。正常对照组大鼠可24 h任意进食,饲喂普通饲料(12%脂肪);高脂饮食组大鼠可24 h任意进食,饲喂高脂饮食(45%脂肪);高脂饮食限时进食组大鼠只在9:00(光照后2 h)至17:00(黑暗前2 h)饲喂高脂饮食。饲养8周后,m Dixon-Quant序列用于评估髌下脂肪垫和右侧腹股沟区皮下脂肪组织的质子密度脂肪分数;ELISA用于定量血清中脂肪因子水平;天狼猩红染色评估髌下脂肪垫纤维化变化;免疫组织化学染色检测解偶联蛋白1在髌下脂肪垫和右侧腹股沟区皮下脂肪组织中的表达以及瘦素、脂联素和肿瘤坏死因子α在髌下脂肪垫中的表达。结果与结论:饲养8周后,(1)与高脂饮食组相比,正常对照组(P=0.036)和高脂饮食限时进食组(P=0.003)的大鼠体质量明显减轻;(2)高脂饮食组大鼠髌下脂肪垫的质子密度脂肪分数明显大于正常对照组(P <0.001)和高脂饮食限时进食组(P=0.004),而3组间皮下脂肪组织的质子密度脂肪分数差异无显著性意义;(3)高脂饮食组的大鼠血清瘦素水平明显高于正常对照组(P=0.030)和高脂饮食限时进食组(P=0.018);(4)与高脂饮食组相比,正常对照组(P <0.001)和高脂饮食限时进食组(P=0.003)以天狼猩红染色为特征的髌下脂肪垫纤维化程度明显降低;(5)高脂饮食组的髌下脂肪垫中瘦素相对表达明显高于正常对照组(P <0.001)和高脂饮食限时进食组(P <0.001);高脂饮食组的髌下脂肪垫中脂联素相对表达明显低于正常对照组(BACKGROUND:Overweight or obesity is the most significant risk factors for knee osteoarthritis.Time-restricted diet shows an effective effect in preventing overweight or obesity.Whether infrapatellar fat pad,an important component of the knee joint,is affected by time-restricted diet and thus influences knee osteoarthritis remains unclear.OBJECTIVE:To employ a high-fat diet-induced rat model to investigate the effect of weight loss by time-restricted diet on infrapatellar fat pad,thereby providing evidence for early prevention and treatment of obesity-related knee osteoarthritis.METHODS:Fifteen male Sprague-Dawley rats were randomly divided into three groups(n=5 per group).Rats in the control group were allowed to eat at will for 24 hours and were fed the defined control diet(12%fat);rats in the high-fat diet group were allowed to eat at will for 24 hours and were fed the high-fat diet(45%fat);and rats in the high-fat diet with time-restricted diet group were fed the high-fat diet only from 9:00(2 hours after the light)to 17:00(2 hours before the dark).After 8 weeks of feeding,mDixon-Quant sequence was used to assess proton density fat fraction in the infrapatellar fat pad and subcutaneous adipose tissue in the right inguinal region.ELISA was used to quantify differences adipokine.Sirius red staining was used to evaluate changes in fibrosis of the infrapatellar fat pad.The expressions of uncoupling protein-1 in the infrapatellar fat pad and subcutaneous adipose tissue in the right inguinal region as well as leptin,adiponectin and tumor necrosis factor-αin the infrapatellar fat pad were detected by immunohistochemistry staining.RESULTS AND CONCLUSION:After 8 weeks of feeding,compared with the high-fat diet group,the body mass of rats in the control group(P=0.036)and the high-fat diet with time-restricted diet group(P=0.003)was significantly reduced.The proton density fat fraction in the infrapatellar fat pad in the high-fat diet group was significantly higher than that in the control group(P<0.001)and the high-fat

关 键 词：限时饮食 髌下脂肪垫 纤维化 肥胖 膝骨关节炎 减重 脂肪因子 动物实验 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364