三种急性胰腺炎大鼠模型的制备及特点比较  

Preparation and characteristics comparison of three acute pancreatitis rat models

作　　者：牛小龙 陈佳靓 郑华群 杨桂媚 姚广涛 Niu Xiaolong;Chen Jialiang;Zheng Huaqun;Yang Guimei;Yao Guangtao(Shanghai Innovation Center of TCM Health Service,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Center for Drug Safety Evaluation and Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学上海中医健康服务协同创新中心,上海市201203 [2]上海中医药大学药物安全评价研究中心,上海市201203

出　　处：《中国组织工程研究》2024年第34期5480-5486,共7页Chinese Journal of Tissue Engineering Research

基　　金：上海市科委生物医药科技支撑专项(22S21901300),项目负责人:姚广涛。

摘　　要：背景:建立稳定可靠的急性胰腺炎动物模型对于了解其发病机制、病理生理学特点及临床用药具有重大意义。国内外研究表明,雨蛙素、L-精氨酸、牛磺胆酸钠均能诱导急性胰腺炎,但是其病理生理学特点、模型特点变化规律尚不明确。目的:采用雨蛙素、L-精氨酸、牛磺胆酸钠制备大鼠急性胰腺炎模型,观察不同时段模型特点变化规律。方法:96只健康雄性SD大鼠随机分为正常组、雨蛙素组、L-精氨组、牛磺胆酸组,每组24只,每组内分为12,24,48 h 3个亚组,每个亚组8只。正常组不做处理,其余3组制备急性胰腺炎模型:雨蛙素组采用雨蛙素6次腹腔注射,间隔1 h;L-精氨酸组采用L-精氨酸2次腹腔注射,间隔1 h;牛磺胆酸钠采用胆胰管逆行注射牛磺胆酸钠诱导急性胰腺炎模型。考察各组大鼠存活率、胰腺大体形态、胰腺脏器指数、淀粉酶、脂肪酶、谷丙转氨酶、谷草转氨酶、尿素氮、肌酐水平,苏木精-伊红染色观察胰腺组织病理特征并进行胰腺损伤评分,评估大鼠不同时段模型特点变化规律。结果与结论:(1)雨蛙素组大鼠总存活率100%,L-精氨酸组为88%,牛磺胆酸钠组为96%;(2)各组模型大鼠胰腺脏器指数均有所升高;雨蛙素组大体观察可见胰腺水肿、分叶模糊、疏松;L-精氨酸组胰腺腺体增大增厚并伴有点片状出血;牛磺胆酸钠胰腺组织出现不同程度充血水肿伴散在点片状出血坏死;(3)各组模型组大鼠血清谷丙转氨酶、谷草转氨酶、尿素氮、肌酐水平与淀粉酶、脂肪酶水平变化一致,雨蛙素组可能在12 h达到峰值(P <0.05),随后呈下降趋势;L-精氨酸24 h达到最高峰(P <0.05),48 h明显下降;牛磺胆酸钠组血清淀粉酶、脂肪酶在12 h维持在较高水平并且有缓慢下降的趋势(P <0.05);(4)与正常组相比,光镜下观察雨蛙素组胰腺腺泡轻度水肿,小叶间隙增宽,炎性细胞较多;L-精氨酸组小叶间隙增宽,大�BACKGROUND:Establishing a stable and reliable animal model of acute pancreatitis is of great significance for understanding its pathogenesis,pathophysiological characteristics,and clinical medication.Domestic and foreign studies have shown that cerulein,L-arginine,and sodium taurocholate can induce acute pancreatitis,but their pathophysiological characteristics and model characteristics are still unclear.OBJECTIVE:To establish an acute pancreatitis rat model using cerulein,L-arginine,and sodium taurocholate and to observe the changing patterns of model features at different time points.METHODS:Ninety-six healthy male Sprague-Dawley rats were randomly divided into normal group,cerulein group,L-arginine group,and sodium taurocholate group,with 24 rats in each group.Within each group,there were three subgroups(n=8 per group):12-,24-,and 48-hour subgroups.Cerulein was administered via intraperitoneal injection six times with a 1-hour interval.L-arginine was administered through two intraperitoneal injections with a 1-hour interval.Sodium taurocholate was injected for inducing acute pancreatitis models through retrograde injection into the bile-pancreatic duct.By examining the rat survival rate,gross morphology of the pancreas,calculating the pancreatic organ index,and measuring levels of amylase,lipase,alanine transaminase,aspartate transaminase,blood urea nitrogen,and creatinine,as well as observing pancreatic tissue pathological features through hematoxylin-eosin staining and conducting a pancreatic injury scoring,we evaluated the changing patterns of model features at different time points.RESULTS AND CONCLUSION:Compared with the normal group,the overall survival rate of rats was 100%in the cerulein group,88%in the L-arginine group,and 96%in the sodium taurocholate group.The pancreatic organ index was increased in all groups.Gross observation indicated that,In the cerulein group,pancreatic edema,blurred lobes,and looseness were visible.In the L-arginine group,the pancreatic glands were enlarged and thickened with

关 键 词：雨蛙素 L-精氨酸 牛磺胆酸钠 急性胰腺炎 动物模型 比较研究 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R332