藜麦CqANS基因的克隆及表达分析  

Cloning and Expression Analysis of CqANS Gene from Chenopodium quinoa

作　　者：张豪杰 陈紫岩 尹航 魏杰 吴传万[1] ZHANG Haojie;CHEN Ziyan;YIN Hang;WEI Jie;WU Chuanwan(Huaiyin Institute of Agricultural Sciences in Xuhuai Area of Jiangsu Province,Huai’an,Jiangsu 223001,China)

机构地区：[1]江苏徐淮地区淮阴农业科学研究所,江苏淮安223001

出　　处：《贵州农业科学》2024年第2期7-14,共8页Guizhou Agricultural Sciences

基　　金：江苏省农业科技自主创新资金项目[CX(22)3188]。

摘　　要：【目的】通过藜麦ANS基因生物信息学分析,研究其蛋白相关特性,为进一步阐明藜麦CqANS基因发挥作用的分子机制提供基础。【方法】通过PCR反应克隆藜麦ANS基因(CqANS)的cDNA全长序列,进而对CqANS编码的蛋白质序列及特征进行预测分析,同时利用实时定量PCR对该基因在不同胁迫处理下的表达进行分析。【结果】藜麦ANS基因包含2个外显子和1个内含子,其编码的蛋白质编码359个氨基酸残基;藜麦CqANS蛋白为亲水性蛋白,不存在信号肽,蛋白质定位于细胞质;其属于PLN03178超家族,具有典型的Fe2+-依赖的双加氧酶家族(2OG-FeII_Oxy)的保守结构域。启动子分析显示在CqANS上游启动子区域存在多种响应非生物胁迫的顺式作用元件。低温胁迫诱导CqANS的表达,而外源ABA处理及干旱处理则抑制CqANS的表达。CqANS与包括AUR62014624-RA在内的多个蛋白存在互作关系。【结论】藜麦ANS基因(CqANS)具有ANS基因家族特征,可能与其他植物的ANS基因存在相似的生物学功能。【Objective】Through bioinformatics analysis of anthocyanidin synthase(ANS)gene in Chenopodium quinoa,its relative protein traits were studied,which provided basis for further studying the molecular mechanism of role of CqANS gene.【Method】The cDNA full-length sequence of C.quinoa ANS gene(CqANS)was cloned by PCR reaction,and then the sequence and characteristics of protein encoded by CqANS were predicted and analyzed.And real-time quantitative PCR was used to analyze the expression of CqANS under different stress treatments.【Result】The CqANS contained two exons and one intron,and the protein encoded by the CqANS encoded 359 amino acid residues.The CqANS was a hydrophilic protein,which had no signal peptide and was located in the cytoplasm.CqANS belonged to the PLN03178 superfamily and had a typical conservative domain of the Fe2+-dependent dioxygenase family(2OG-FeII_Oxy).Promoter analysis showed that there were many cis-acting elements in the promoter region of CqANS in response to abiotic stress.Low temperature stress could induce the expression of CqANS,while exogenous ABA treatment and drought treatment could inhibit the expression of CqANS.CqANS interacted with several proteins,including AUR62014624-RA.【Conclusion】The ANS gene of C.quinoa(CqANS)has the characteristics of ANS gene family and may has similar biological functions as ANS genes in other plants.

关 键 词：藜麦 ANS 生物信息学分析 基因定位 表达分析 

分 类 号：S519[农业科学—作物学]