喜树果黄酮绿色提取工艺的响应面法优化  

Optimization of the Green Extraction Process ofCamptotheca Acuminata Flavonoids by Response Surface Methodology

作　　者：张欣欣 范圆圆 原甜梦 刘雨露 肖莉[1] ZHANG Xinxin;FAN Yuanyuan;YUAN Tianmeng;LIU Yulu;XIAO Li(School of Life Sciences,Huzhou University,Huzhou 313000,China)

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《湖州师范学院学报》2024年第2期47-52,共6页Journal of Huzhou University

基　　金：浙江省大学生创新创业训练项目(S202310347024)。

摘　　要：采用绿色低共熔溶剂(DES)优化喜树果中黄酮成分的提取工艺.合成12种DES溶剂,通过单因素结合Box-Behnken响应面法优化提取工艺,从中筛选出提取率最高的DES组合.结果表明,最优工艺条件为:液料比为61∶1、氯化胆碱与柠檬酸的摩尔比为2∶3、含水量为20%、提取时间为51 min.该工艺的喜树果总黄酮提取率达3.29%,是传统有机溶剂提取法的3倍.该研究为喜树果的开发利用提供了参考,也为中药材活性成分的绿色提取提供了一定的借鉴.The extraction process of Fructus Camptothecae was optimized by using green deep eutectic solvents(DESs).Twelve kinds of DESs were synthesized,and the highest extraction rate of the DESs combination were screened by using a single factor combined with Box-Behnken response surface method extraction process.The results showed that the optimal process conditions were liquid-to-material ratio of 61∶1,molar ratio of choline chloride to citric acid of 2∶3,water content 20%,extraction time 51 minutes.The extraction rate of total flavonoids from Camptotheca acaciae reached 3.29%,which was 3 times of the traditional organic solvent extraction method.This study laid the foundation for the development and utilization of Camptotheca acuminata,and also provided certain reference for the green extraction of active ingredients of Chinese medicinal materials.

关 键 词：低共熔溶剂 喜树果 黄酮 绿色提取 

分 类 号：S476[农业科学—农业昆虫与害虫防治]