大别班达病毒的遗传进化机制研究  

Investigation of the genetic evolutionary mechanisms of Dabie bandavirus

作　　者：张宇涵 李超 王玉昊 韦雪敏 许一菲 ZHANG Yu-han;LI Chao;WANG Yu-hao;WEI Xue-min;XU Yi-fei(Department of Microbiology,School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China;Suzhou Research Institute of Shandong University,Suzhou 215123,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院微生物检验学系,济南250012 [2]山东大学苏州研究院,苏州215123

出　　处：《中国人兽共患病学报》2024年第2期171-178,共8页Chinese Journal of Zoonoses

基　　金：山东省优秀青年科学基金项目(海外)(No.2022HWYQ-056);江苏省自然科学青年基金(No.BK20220270)联合资助。

摘　　要：目的阐明大别班达病毒(Dabie bandavirus,DBV)的基因型流行分布特征,探究DBV进化机制的动态变化,为DBV的预防控制提供科学依据。方法使用Iqtree软件以最大似然法构建DBV的L、M、S片段系统发育进化树并进行遗传进化关系的分析;以系统发育进化树为参考,筛选重配株;使用RDP4软件进行DBV重组情况的检测;DBV序列的遗传距离以及选择压力分析由Mega11.0软件计算。结果重组分析显示共88株毒株出现重组现象,潜在重组事件共计98个。L、M、S片段的重组率分别为3.48%、2.88%、1.75%。重组事件在非人类宿主中检测到的比例相较之前的研究有所上升。经重配分析,在148株重配株中发现了36种重配基因型,未报道过的重配基因型有16种。本研究检测到24株同时发生重组和重配的基因组序列。dN/dS的结果表明,RNA依赖的RNA聚合酶(RdRp)、糖蛋白(GP)、核蛋白(NP)和非结构蛋白(NSs)进化过程中可能主要受负选择压力影响。结论越来越多的动物宿主如狗、猫、牛等参与DBV的重组、重配事件中,使其进化机制更趋复杂,这对DBV疫苗的设计和SFTS的预防带来了更大的挑战。The purpose of this study was to elucidate the genotype epidemic distribution characteristics of Dabie bandavirus(DBV)and study the changes in mechanisms of DBV evolution,to provide a scientific basis for disease prevention and control.DBV phylogenetic trees were constructed with Iqtree.Recombination events were detected with Recombination Detection Program v4.101(RDP4),and reassortants were screened according to the phylogenetic evolutionary tree.Genetic distances and selection pressure analysis of DBV sequences were calculated in Mega11.0 software.A total of 98 potential recombination events in 88 strains were detected.The recombination rates of the L,M,and S fragments were 3.48%,2.88%,and 1.75%,respectively.The proportion of recombination events detected in non-human hosts was greater than observed in previous studies.A total of 36 reassortment forms in 148 reassortants were identified,and 24 sequences with simultaneous recombination and rearrangement were discovered.The dN/dS results suggested that negative selection pressure might be the main selection pressure on the L,M,and S segments.The evolutionary mechanism of DBV is complicated,because an increasing number of animal hosts are involved in its recombination and reassortment events,thus posing challenges in the design of DBV vaccines and the prevention of SFTS.

关 键 词：DBV 重组 重配 选择压力 进化 

分 类 号：R373.3[医药卫生—病原生物学]