锰纳米酶比色法检测电化学制备过氧化氢  

Manganese Nanozyme Based Colorimetric Detection of Hydrogen Peroxide Prepared by Electrochemistry

作　　者：董旭阳 李哲 夏明远 康革 袁振峰 逯一中 DONG Xuyang;LI Zhe;XIA Mingyuan;KANG Ge;YUAN Zhenfeng;LU Yizhong(School of Materials Science and Engineering,University of Jinan,Jinan 250022,Shandong,China)

机构地区：[1]济南大学材料科学与工程学院,山东济南250022

出　　处：《济南大学学报（自然科学版）》2024年第2期210-218,共9页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金项目(22172063);泰山青年学者专家计划(tsqn201812080);济南市创新团队自主培养计划项目(2021GXRC052)。

摘　　要：为了拓展纳米酶的应用,采用离子交换和高温热解法将均匀分散的锰原子掺杂到中空碳骨架中制备中空结构的锰纳米酶;提出一种基于锰纳米酶的比色法,用于检测电化学双电子氧还原原位产生的过氧化氢的浓度。结果表明,锰纳米酶具有优异的类过氧化物酶活性,可催化过氧化氢将无色3, 3’, 5, 5’-四甲基联苯胺(TMB)氧化为蓝色氧化态TMB;当过氧化氢浓度为0~6 mmol/L时,体系的吸光度与过氧化氢浓度具有良好的多段线性关系,锰纳米酶比色法具有极低的检出限(0.45μmol/L)和优异的抗干扰性能。To expand the application of nanozymes,homogeneously dispersed manganese atoms were doped into the hollow carbon skeleton by ion exchange and high temperature pyrolysis,and a hollow manganese nanozyme(Mn-NC)was prepared.A colorimetric method based on Mn-NC was developed to detect the concentration of hydrogen peroxide pro-duced in situ by electrochemical two-electron oxygen reduction.The results show that Mn-NC has excellent peroxidase like activity and can catalyze the oxidation of colorless 3,3’,5,5’-tetramethylbenzidine(TMB)to blue oxidized TMB by hydrogen peroxide.When the hydrogen peroxide concentration is 0~6 mmol/L,the absorbance of the system has a good multi-segment linear relationship with the hydrogen peroxide concentration,and Mn-NC colorimetric method has very low detection limit(0.45μmol/L)and excellent anti-interference performance.

关 键 词：电化学 纳米酶 比色分析 过氧化氢 

分 类 号：Q554.6[生物学—生物化学] O657.32[理学—分析化学]