Brain circuits for retching-like behavior  

作　　者：Lifang Huo Zhimin Ye Meiling Liu Ziqing He Meizhu Huang Dapeng Li Qian Wu Qian Wang Xiaoqun Wang Peng Cao Ji Dong Congping Shang 

机构地区：[1]School of Basic Medical Sciences,Guangzhou National Laboratory,The Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou 510799,China [2]Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou 510320,China [3]Department of Neurobiology,School of Basic Medical Sciences,Beijing Key Laboratory of Neural Regeneration and Repair,Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing 100069,China [4]State Key Laboratory of Cognitive Neuroscience and Learning,IDG/McGovern Institute for Brain Research,Beijing Normal University,Beijing 100875,China [5]Changping Life Science Laboratory,Beijing 102299,China [6]National Institute of Biological Sciences,Beijing 102206,China

出　　处：《National Science Review》2024年第1期140-155,共16页国家科学评论（英文版）

基　　金：supported by the National Key R&D Program of China(STI2030-Major Projects-2021ZD0202400 to C.S.);the National Natural Science Foundation of China(32271069to C.S.);the Research Foundation for Advanced Talents from Guangzhou National Laboratory(YW-JCYJ0603 to C.S.);Bioland Laboratory(1102101217 to C.S.);the Young Elite Scientist Sponsorship Program by CAST(YESS20200122 to C.S.);the China Postdoctoral Science Foundation fellowship(PDXM29424801 to L.H.)。

摘　　要：Nausea and vomiting are important defensive responses to cope with pathogens and toxins that invade the body.The nucleus of the solitary tract(NTS)is important for initiating these responses.However,the molecular heterogeneities and cellular diversities of the NTS occlude a better understanding of these defensive responses.Here,we constructed the single-nucleus transcriptomic atlas of NTS cells and found multiple populations of NTS neurons that may be involved in these defensive responses.Among these,we identified Calbindin1-positive(Calb1^(+))NTS neurons that are molecularly distinct from Tac1^(+)neurons.These Calb1^(+)neurons are critical for nausea and retching induced by cereulide;an emetic toxin secreted by Bacillus Cereus.Strikingly,we found that cereulide can directly modulate vagal sensory neurons that innervate Calb1^(+)NTS neurons,a novel mechanism distinct from that for nausea and retching induced by Staphylococcal enterotoxin A.Together,our transcriptomic atlas of NTS neurons and the functional analyses revealed the neural mechanism for cereulide-induced retching-like behavior.These results demonstrate the molecular and cellular complexities in the brain that underlie defensive responses to the diversities of pathogens and toxins.

关 键 词：VOMITING NAUSEA nucleus of the solitary tract Calbindin1 neural circuits 

分 类 号：R338[医药卫生—人体生理学]