Lipid phosphorylation by a diacylglycerol kinase suppresses ABA biosynthesis to regulate plant stress responses  

作　　者：Jianwu Li Shuaibing Yao Sang-Chul Kim Xuemin Wang 

机构地区：[1]Department of Biology,University of Missouri-St.Louis,St.Louis,MO 63121,USA [2]Donald Danforth Plant Science Center,St.Louis,MO 63132,USA

出　　处：《Molecular Plant》2024年第2期342-358,共17页分子植物（英文版）

基　　金：Research reported in this article was supported by the National Institute of General Medical Sciences of the National Institutes of Health under award number R01GM141374 and the National Science Foundation grants 2222157 and 2302424.

摘　　要：Lipid phosphorylation by diacylglycerol kinase(DGK)that produces phosphatidic acid(PA)plays important roles in various biological processes,including stress responses,but the underlying mechanisms remain elusive.Here,we show that DGK5 and its lipid product PA suppress ABA biosynthesis by interacting withABA-DEFICIENT2(ABA2),a key ABA biosynthesis enzyme,to negatively modulate plant responseto abiotic stress tested in Arabidopsis thaliana.Loss of DGK5 function rendered plants less damaged,whereas overexpression(OE)of DGK5 enhanced plant damage to water and salt stress.The dgk5 mutant plants exhibited decreased total cellular and nuclear levels of PA with increased levels of diacylglycerol,whereas DGK5-OE plants displayed the opposite effect.Interestingly,we found that both DGK5 and PA bind to the ABA-synthesizing enzyme ABA2 and suppress its enzymatic activity.Consistently,the dgk5 mutant plants exhibited increased levels of ABA,while DGK5-OE plants showed reduced ABA levels.In addition,we showed that both DGK5 and ABA2 are detected in and outside the nuclei,and loss of DGK5 function decreased the nuclear association of ABA2.We found that both DGK5 activity and PA promote nuclear association of ABA2.Taken together,these results indicate that both DGK5 and PA interact with ABA2 to inhibit its enzymatic activity and promote its nuclear sequestration,thereby sup-pressing ABA production in response to abiotic stress.Our study reveals a sophisticated mechanism by which DGK5 and PA regulate plant stress responses.

关 键 词：diacylglycerol kinase phosphatidic acid DIACYLGLYCEROL lipid signaling stress responses lipid-protein binding protein-protein interaction Arabidopsis 

分 类 号：Q945.78[生物学—植物学]