梅花鹿源大肠杆菌ESBLs基因型检测与分析  

Detection and Analysis of ESBLs Genotype in Escherichia coli from Sika Deer

作　　者：姚茵茵 聂瑞欣 周航[1] 薛原[1] YAO Yinyin;NIE Ruixin;ZHOU Hang;XUE Yuan(College of Wildlife and Protected Area,Northeast Forestry University,Harbin Heilongjiang 150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,黑龙江哈尔滨150040

出　　处：《现代畜牧科技》2024年第2期19-21,共3页Modern Animal Husbandry Science & Technology

基　　金：大学生创新训练项目(202310225523)。

摘　　要：为分析黑龙江省梅花鹿源产超广谱β-内酰胺类酶(ESBLs)大肠杆菌耐药基因的分布情况,为临床抗微生物药的合理使用提出指导意见。从黑龙江省4个养殖场中分离出130株梅花源大肠杆菌,采用K-B纸片法,结果显示对诺氟沙星、环丙沙星、氧氟沙星的耐药率达到10%以上。采用PCR技术对ESBLs基因进行检测。结果显示,耐药基因OXA、TEM的检出率分别为13.85%(18/130)、76.15%(99/130),CTX-M和SHV均未检出。4个养殖场的ESBLs基因以TEM为主。In order to analyze the distribution of drug resistance genes of extended spectrum beta lactamases(ESBLs)producing Escherichia coli from sika deer in Heilongjiang Province,and put forward suggestions on rational use of Antimicrobial Drugs.130 sika deer-derived Escherichia coli strains were screened from 4 farms in Heilongjiang Province,and Kirby-Bauer disk diffusion method was used.The results showed that the drug resistance rate to norfloxacin,ciprofloxacin and ofloxacin reached mohan 10%.The ESBLs gene was detected by PCR technique.The results showed that the detection rates of OXA and TEM were 13.85%(18/130)and 76.15%(99/130),respectively.CTX-M and SHV were not detected.TEM was the dominant ESBLs gene in 4 farms.

关 键 词：梅花鹿 ESBLS 大肠杆菌 耐药表型 

分 类 号：S858.25[农业科学—临床兽医学]