1株边鸡源产蛋白酶枯草芽孢杆菌的分离鉴定与生物学特性研究  

Isolation,Identification and Biological Characteristics of Protease-Producing Bacillus subtilis from Bian Chicken

作　　者：孙丽萍 胡馨元 钮慧晶 王琴 裴彩霞[1] 李毅 夏呈强 SUN Liping;HU Xinyuan;NIU Huijing;WANG Qin;PEI Caixia;LI Yi;XIA Chengqiang(College of Animal Science,Shanxi Agricultural University,Taigu Shanxi 030801,China;College of Life Sciences,Shanxi Agricultural University,Taigu Shanxi 030801,China)

机构地区：[1]山西农业大学动物科学学院,山西太谷030801 [2]山西农业大学生命科学学院,山西太谷030801

出　　处：《现代畜牧科技》2024年第2期33-38,共6页Modern Animal Husbandry Science & Technology

基　　金：山西省优秀博士来晋工作奖励项目(SXYBKY2019024,SXYBKY2019025);山西农业大学科技创新基金项目(2020BQ07);山西省重点研发计划项目(202102140601019)。

摘　　要：该试验旨在筛选具有高产蛋白酶能力的益生芽孢杆菌,扩大微生态制剂候选菌株资源。以边鸡直肠内容物为分离来源,经脱脂奶粉平板初筛获得16株产蛋白酶菌株,液体发酵复筛后,选取1株蛋白酶活力高达119.7 U/mL的芽孢杆菌FRE76进行后续研究。通过16S rRNA基因测序与进化树分析,并结合形态学与生理生化鉴定,判定该菌株为枯草芽孢杆菌。病原菌抑制试验表明,该菌对大肠杆菌和金黄色葡萄球菌均有明显的抑制作用。耐酸和耐胆盐检测发现,在pH值3.0和4.0时该菌株存活率均为50%以上,在胆盐浓度为0.1%和0.3%时菌株存活率均能达到60%以上。该试验筛选获得的枯草芽孢杆菌FRE76,具有优良的蛋白酶生产性能、较好的致病菌抑制能力和较强的抗逆性,是一株潜在的益生菌菌株。The purpose of this experiment was to screen probiotic with high protease-producing capacity and to expand the candidate strain resources of microecological agents.In this experiment,16 protease-producing strains were obtained by plate screening of skimmed milk using the rectal contents of Bian chickens as the isolation source,and after rescreened of liquid fermentation,one strain named FRE76 with a protease activity of 119.7 U/mL was selected for further study.Based on the 16S rRNA gene sequencing and evolutionary tree analysis,combined with morphological and physiological and biochemical identification,the strain was identified as Bacillus subtilis.The pathogens inhibition test showed that Bacillus subtilis had a significant inhibitory effect on both Escherichia coli and Staphylococcus aureus.Acid and bile salt resistance tests showed that the survival rate of this strain was more than 50% at pH 3.0 and 4.0,and the survival rate of the strain could reach more than 60% at bile salt concentrations of 0.1% and 0.3%.In summary,it can be found that Bacillus subtilis FRE76 screened in this experiment possessed excellent property for protease production,good inhibitory ability of pathogens and strong stress resistance,and is a potential probiotic strain.

关 键 词：益生菌 枯草芽孢杆菌 分离鉴定 蛋白酶 生物学特性 

分 类 号：S831.5[农业科学—畜牧学]