唐山地区奶牛临床型乳腺炎源葡萄球菌的分离鉴定  

Isolation and Identification of Staphylococcus Aureus from Clinical Mastitis in Dairy Cows in Tangshan Area

作　　者：李春芳[1] 陈彦丽 刘志勇[1] 张晓利[1] LI Chunfang;CHEN Yanli;LIU Zhiyong;ZHANG Xiaoli(Tangshan Animal Epidemic Prevention and Control Center,Tangshan Hebei 063000,China;Tangshan Academy of Agricultural Sciences,Tangshan Hebei 063000,China)

机构地区：[1]河北省唐山市动物疫病预防控制中心,河北唐山063000 [2]河北省唐山市农业科学研究院,河北唐山063000

出　　处：《现代畜牧科技》2024年第2期47-49,共3页Modern Animal Husbandry Science & Technology

基　　金：河北省三期现代农业产业技术体系奶牛产业创新团队唐山综合试验推广站(HBCT2023180403)。

摘　　要：目的:分离和鉴定河北省唐山地区奶牛临床型乳腺炎源葡萄球菌,并了解其药敏情况。方法:选择河北省唐山地区某规模化大型奶牛养殖场发生的60例临床型乳腺炎源葡萄球菌病的奶牛乳汁作为临床样本,进行病原菌分离鉴定、革兰氏染色镜检、PCR扩增鉴定和药敏试验。结果:共分离培养获得金黄色葡萄球菌和产色金黄色葡萄球菌2种类型的葡萄球菌,呈革兰氏阳性。PCR检测可扩增出一大小为1459 bp的特异性条带,分离培养的2株葡萄球菌的蔗糖、麦芽糖、N-乙酰葡萄糖胺、甘露糖、果糖、甘露醇、尿素、精氨酸双水解、硝酸盐还原试验为阳性。金黄色葡萄球菌对克林霉素、红霉素、氧氟沙星、头孢噻吩、四环素5种抗菌药物敏感,产色葡萄球菌除了红霉素外,其余11种抗菌药物均敏感。结论:该研究为提高奶牛临床型乳腺炎源葡萄球菌病的治疗效果奠定基础,也为今后本地区奶牛乳腺炎的科学防治提供参考价值。Objective:To isolate and identify Staphylococcus aureus from clinical mastitis of cows in Tangshan area,Hebei Province,and to understand its drug sensitivity.Method:The 60 cases of clinical mastitis related Staphylococcus aureus disease in a large-scale dairy farm in Tangshan,Hebei Province were selected as clinical samples for pathogen isolation and identification,Gram staining microscopy,PCR amplification identification,and drug sensitivity testing.Result:The results showed that two types of Staphylococcus aureus,Staphylococcus aureus and Staphylococcus aureus chromogene,were co isolated and cultured,and they were Gram positive.PCR detection can amplify a specific band with a size of 1459 bp.The sucrose,maltose,N-acetylglucosamine,mannose,fructose,mannitol,urea,arginine double hydrolysis,and nitrate reduction tests of the two strains of Staphylococcus isolated and cultured were positive.Staphylococcus aureus is sensitive to five antibiotics,including clindamycin,erythromycin,ofloxacin,cefotaxime,and tetracycline.Except for erythromycin,all 11 other antibiotics are sensitive to Staphylococcus aureus chromogenes.Conclusion:This study lays the foundation for improving the therapeutic effect of clinical mastitis associated Staphylococcus aureus in cows,and also provides reference value for the scientific prevention and treatment of mastitis in local cows in the future.

关 键 词：奶牛 临床型乳腺炎 葡萄球菌 分离鉴定 

分 类 号：S858.23[农业科学—临床兽医学]