Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system  

作　　者：Yating Yi Youqi Li Shiwen Zhang Yi Men Yuhong Wang Dian Jing Jiayi Ding Qingjie Zhu Zexi Chen Xingjun Chen Jun-Liszt Li Yilong Wang Jun Wang Hanchuan Peng Li Zhang Wenjing Luo Jian Q.Feng Yongwen He Woo-Ping Ge Hu Zhao 

机构地区：[1]State Key Laboratory of Oral Diseases,National Center for Stomatology,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China [2]Department of Orthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China [3]Chinese Institute for Brain Research,Beijing,China [4]Department of Neurology,Beijing Tiantan Hospital,Capital Medical University,Beijing,China [5]School of Basic Medical Sciences,Capital Medical University,Beijing,China [6]Department of Oral Implantology,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China [7]Department of Head and Neck Oncology,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan,China [8]West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu,Sichuan,China [9]Department of Orthodontics,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai,China [10]Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,China [11]SEU-ALLEN Joint Center,Institute for Brain and Intelligence,Southeast University,Nanjing,Jiangsu,China [12]341 Hunnewell St Needham,MA,USA [13]Texas A&M University,College of Dentistry,Dallas,TX,USA [14]Qujing Medical College,Qujing,Yunnan,China

出　　处：《Cell Research》2024年第2期124-139,共16页细胞研究（英文版）

基　　金：supported by a startup fund from the Chinese Institute for Brain Research to H.Z.and W.-P.G.,the National Natural Science Foundation of China(82101059 to Y.Y.);the Research Funding from West China School/Hospital of Stomatology Sichuan University(RCDWJS2022-11 to Y.Y.).

摘　　要：Achieving uniform optical resolution for a large tissue sample is a major challenge for deep imaging.For conventional tissue clearing methods,loss of resolution and quality in deep regions is inevitable due to limited transparency.Here we describe the Transparent Embedding Solvent System(TESOS)method,which combines tissue clearing,transparent embedding,sectioning and block-face imaging.We used TESOS to acquire volumetric images of uniform resolution for an adult mouse whole-body sample.The TESOS method is highly versatile and can be combined with different microscopy systems to achieve uniformly high resolution.With a light sheet microscope,we imaged the whole body of an adult mouse,including skin,at a uniform 0.8×0.8×3.5μm^(3) voxel resolution within 120 h.With a confocal microscope and a 40×/1.3 numerical aperture objective,we achieved a uniform sub-micron resolution in the whole sample to reveal a complete projection of individual nerve axons within the central or peripheral nervous system.Furthermore,TESOS allowed the first mesoscale connectome mapping of individual sensory neuron axons spanning 5 cm from adult mouse digits to the spinal cord at a uniform sub-micron resolution.

关 键 词：EMBEDDING TRANSPARENT DIGIT 

分 类 号：R338[医药卫生—人体生理学]