The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome–lysosome fusion  

作　　者：Fenglei Jian Shen Wang Rui Tian Yufen Wang Chuangpeng Li Yan Li Shixuan Wang Chao Fang Cong Ma Yueguang Rong 

机构地区：[1]School of Basic Medicine,Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease,Huazhong University of Science and Technology,Wuhan,Hubei,China [2]Key Laboratory of Molecular Biophysics of the Ministry of Education,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan,Hubei,China [3]Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,China [4]Cell Architecture Research Center,Huazhong University of Science and Technology,Wuhan,Hubei,China

出　　处：《Cell Research》2024年第2期151-168,共18页细胞研究（英文版）

基　　金：supported by grants from NSFC (92254302, 91854116, 32170685 and 31771529 to Y.R.);supported by the Fundamental Research Funds for the Central Universities (5003510089 and 2023BR028 to Y.R.).

摘　　要：Autophagosome–lysosome fusion mediated by SNARE complexes is an essential step in autophagy.Two SNAP29-containing SNARE complexes have been extensively studied in starvation-induced bulk autophagy,while the relevant SNARE complexes in other types of autophagy occurring under non-starvation conditions have been overlooked.Here,we found that autophagosome–lysosome fusion in selective autophagy under non-starvation conditions does not require SNAP29-containing SNARE complexes,but requires the STX17-SNAP47-VAMP7/VAMP8 SNARE complex.Further,the STX17-SNAP47-VAMP7/VAMP8 SNARE complex also functions in starvation-induced autophagy.SNAP47 is recruited to autophagosomes following concurrent detection of ATG8s and PI(4,5)P2 via its Pleckstrin homology domain.By contrast,SNAP29-containing SNAREs are excluded from selective autophagy due to inactivation by O-GlcNAcylation under non-starvation conditions.These findings depict a previously unknown,default SNARE complex responsible for autophagosome–lysosome fusion in both selective and bulk autophagy,which could guide research and therapeutic development in autophagy-related diseases.

关 键 词：conditions. complex. domain. 

分 类 号：Q2[生物学—细胞生物学]