Effects of Inoculation with Phosphate Solubilizing Bacteria on the Physiology,Biochemistry,and Expression of Genes Related to the Protective Enzyme System of Fritillaria taipaiensis P.Y.Li  

作　　者：Zhifen Shi Fumei Pan Xiaotian Kong Jiaqi Lang Mingyan Ye Qian Wu Guangzhi Wang Liang Han Nong Zhou 

机构地区：[1]College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,611130,China [2]College of Pharmacy,Dali University,Dali,671000,China [3]College of Food and Biological Engineering,Chongqing Three Gorges University,Chongqing,404120,China [4]Diqing Tibetan Autonomous Prefecture Institute of Plateau Biology,Diqing,674400,China [5]Chongqing Institute of Medicinal Plant Cultivation,Chongqing,408435,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第2期247-260,共14页国际实验植物学杂志（英文）

基　　金：This work was funded by Chongqing Municipal Technology Innovation and Application Development Program(cstc2020jscx-gksb0001);Yunnan Academician(Expert)Workstation Project(202105AF150073).

摘　　要：Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highes

关 键 词：Fritillaria taipaiensis P.Y.Li phosphate solubiliozing bacteria photosynthesis physiology and biochemistry protective enzymes 

分 类 号：Q94[生物学—植物学]