HPLC法检测6%吡嘧磺隆·丙草胺·二氯喹啉酸颗粒剂  

Determination of 6%Pyrazosulfuron-ethyl,Pretilachlor and Quinclorac by HPLC

作　　者：张平 祁月月 杨明君 徐勇存 金鑫雷 ZHANG Ping;QI Yueyue;YANG Mingjun;XU Yongcun;JIN Xinlei*(Testing Center of Zhejiang Tianfeng Bioscience Co.,Ltd.,Jinhua 321000,China)

机构地区：[1]浙江天丰生物科学有限公司检测中心,浙江金华321000

出　　处：《化工管理》2024年第7期43-45,共3页Chemical Engineering Management

摘　　要：采用高效液相色谱法,使用50 mm×4.6 mm C_(18)色谱柱,以甲醇+乙腈+0.05%磷酸水溶液为流动相,在254 nm(2 min后更改为230 nm)波长下对吡嘧磺隆·丙草胺·二氯喹啉酸颗粒剂进行分离和检测。经检测二氯喹啉酸、吡嘧磺隆、丙草胺在此分析方法下具有良好的线性关系。吡嘧磺隆线性回归方程为y=3144x+2.8726,相关系数R^(2)=0.9999。丙草胺线性回归方程为y=426.78x-0.08,相关系数R^(2)=0.9994。二氯喹啉酸线性回归方程为y=5630.4x+51.736,相关系数R^(2)=1.0000。此检测方法具有线性关系好、精密度高、回收率高、检测速度快的特点,适用于该产品的定性定量检测。The HPLC method was used to separate and determine the contents of pyrazosulfuron-ethyl,pretilachlor and ouinclorac in the pesticiside granule which contained the active ingredients mentioned above.In the method,the column(50 mm×4.6 mm C_(18))was used and the mixture of acetonittrile,methanol and 0.05%phosphoric acid solution was used as the mobile phase,and 254nm was the selected wavelength in the determination(the selected wavelength was changed 230 nm 2 min later).The method was validated also and the linear equation of the method for pyrazosulfuron-ethyl is y=3144x+2.8726,the linear correlation coefficient is R^(2)=0.9999;the linear equation of the method for pretilachlor is y=426.78x-0.08,the linear correlation coefficient is R^(2)=0.9999;the linear equation of the method for quinclorac is y=5630.4x+51.736,the linear correlation coefficient is R^(2)=1.0000,The validation results show the following characteristics of the method:a good linear relationship,high precision,high recovery and good resolution.The method is suitable for qualitative and quantitative detection.

关 键 词：二氯喹啉酸 吡嘧磺隆 丙草胺 高效液相色谱法 定性定量检测 

分 类 号：TQ450[化学工程—农药化工]