基于体内外抗氧化作用的菊花对自发性高血压大鼠血压的影响  

Effects of Chrysanthemi Flos on blood pressure of spontaneously hypertensive rats based on its antioxidant activity in vitro and in vivo

作　　者：卢新 张留记[1,2] 李开言 屠万倩 王建霞 LU Xin;ZHANG Liu-ji;LI Kai-yan;TU Wan-qian;WANG Jian-xia(School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450008,Henan Province,China;Henan Engineering Center of Genuine Medicinal Materials Comprehensive Development,Henan Provincial Integrated Hospital of Traditional Chinese Medicine and Western Medicine,Zhengzhou 450004,Henan Province,China;Academic Affairs Office,Henan Health Care College,Zhengzhou 450008,Henan Province,China)

机构地区：[1]河南中医药大学药学院,河南郑州450008 [2]河南省中西医结合医院,河南省道地药材综合开发工程研究中心,河南郑州450004 [3]河南卫生健康干部学院教务处,河南郑州450008

出　　处：《中国临床药理学杂志》2024年第4期564-568,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河南省科技厅工程技术研究中心建设基金资助项目(2020338)。

摘　　要：目的 研究菊花对自发性高血压大鼠(SHR)血压的影响和体内外的抗氧化活性。方法 将SHR随机分为模型组、对照组和低、高剂量实验组,每组10只;另取10只WKY大鼠为空白组。高、低剂量组分别灌胃给予2.10和0.525 g·kg^(-1)菊花提取物;对照组灌胃给予5.25 mg·kg^(-1)氯沙坦;空白组和模型组均灌胃给予相同体积的0.9%NaCl溶液。5组大鼠每天灌胃给药1次,连续灌胃8周。观察各组大鼠收缩压(SBP)和舒张压(DBP)的变化,用试剂盒比色法测定血清中过氧化氢酶(CAT)、超氧歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH)和丙二醛(MDA)含量,用1,1-二苯基-2-苦基肼(DPPH)法和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法测定菊花提取物对体外自由基的清除率。结果 空白组、模型组、对照组和高、低剂量实验组大鼠的SBP分别为(132.00±2.45)、(204.00±4.55)、(171.00±2.16)、(181.00±3.74)和(184.67±4.78)mmHg,DBP分别为(73.33±4.03)、(175.67±3.40)、(120.33±0.94)、(125.33±2.87)和(125.67±2.36)mmHg,CAT含量分别为(9.24±3.99)、(8.40±2.98)、(9.24±2.42)、(8.59±2.70)和(8.49±1.47)U·mL^(-1),SOD含量分别为(122.40±12.30)、(75.30±28.37)、(125.39±31.35)、(110.92±26.14)和(103.37±22.31)U·mL^(-1),GSH含量分别为(117.93±10.18)、(78.29±23.68)、(118.57±26.08)、(109.89±20.52)和(98.73±14.71)U·mL^(-1),MDA含量分别为(8.36±2.08)、(8.45±3.38)、(8.22±3.04)、(7.09±3.21)和(7.24±3.32)nmol·L^(-1)。模型组的上述指标与低、高剂量实验组和对照组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。菊花提取物具有较好的体外清除DPPH和ABTS自由基能力,最高清除率分别为90.29%和92.67%。结论 菊花提取物具有较好降低血压的效果,抗氧化可能是其降压作用的机制之一。Objective To study the effects of Chrysanthemum Flos on blood pressure of spontaneously hypertensive rats(SHR) and evaluate its antioxidant activity in vitro and in vivo.Methods SHR were randomly divided into model,control and experimental-L,-H groups with 10rats per group,and 10 WKY rats as blank group.Experimental-H,-L groups were given 2.10 and 0.525 g · kg^(-1) Chrysanthemum Flos extract by gavage;control group received 5.25 mg · kg^(-1) losartan by gavage;blank and model groups were given the same volume of 0.9%NaCl solution by gavage.Rats in each group were gavaged once a day for8 weeks.After 8 weeks of continuous intragastric administration,the systolic blood pressure(SBP) and diastolic blood pressure(DBP) were observed.The contents of catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH) and malondialdehyde(MDA) in serum were measured with kit colorimetry method.The in vitro free radical scavenging rates of Chrysanthemum Flos extract were detected by 1,1-diphenyl-2-picrylhydrazyl(DPPH) and2,2 '-amino-di(2-ethyl-benzothiazoline sulphonic acid-6) ammonium salt(ABTS) methods.Results The SBP of blank,model,control and experimental-H,-L groups were(132.00±2.45),(204.00±4.55),(171.00±2.16),(181.00±3.74) and(184.67±4.78) mmHg;the DBP were(73.33±4.03),(175.67±3.40),(120.33±0.94),(125.33±2.87) and(125.67±2.36) mmHg;the contents of serum CAT were(9.24±3.99),(8.40±2.98),(9.24±2.42),(8.59±2.10) and(8.49±1.47) U·mL^(-1);the contents of serum SOD were(122.40±12.30),(75.30±28.37),(125.39±31.35),(110.92±26.14) and(103.37±22.31)U·mL^(-1);the contents of serum GSH were(117.93±10.18),(78.29±23.68),(118.57±26.08),(109.89±20.52) and(98.73±14.71) U·mL^(-1);the contents of serum MDA were(8.36±2.08),(8.45±3.38),(8.22±3.04),(7.09±3.21) and(7.24±3.32) nmol·L^(-1),respectively.Compared with model group,the differences of above indicators in control group andexperimental-H,-L groups were statistically significant(P <0.05,P <0.01).Chrysanthemum Flos extract showed certain free radical

关 键 词：菊花 自发性高血压 降压 抗氧化 体内外 

分 类 号：R28[医药卫生—中药学]