基于盲肠内容物代谢组学的炙红芪干预脾气虚大鼠的作用研究  

Honey-processed Hedysari Radix in treatment of spleen-Qi deficiency rats based on metabonomics of the cecum contents

作　　者：孙宇靖 宋沁洁 王彦钧 边甜甜 张育贵 李咸慰 李国峰 李越峰 SUN Yu-jing;SONG Qin-jie;WANG Yan-jun;BIAN Tian-tian;ZHANG Yu-gui;LI Xian-wei;LI Guo-feng;LI Yue-feng(Scientific Research and Experimental Center,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学科研实验中心,甘肃兰州730000 [2]甘肃中医药大学药学院,甘肃兰州730000

出　　处：《中国临床药理学杂志》2024年第4期584-588,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81960713,82160750,81460611);甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21);甘肃省中药制药工艺工程研究中心开放课题基金资助项目(ZYGY202003);甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569)。

摘　　要：目的 以脾气虚大鼠的盲肠内容物为研究对象,筛选炙红芪干预脾气虚大鼠肠道功能的潜在生物标记物,分析其代谢途径。方法 将60只SD雄性大鼠随机分为空白组、模型组、实验组和对照组,除空白组外,其余大鼠均用三因素复合造模法建立脾气虚模型。实验组灌胃给予12.60 g·kg^(-1)蜜炙红芪水提物;对照组灌胃给予0.63 g·kg^(-1)双歧杆菌乳杆菌三联活菌片混悬液;空白组和模型组均灌胃给予等剂量蒸馏水。检测动物体质量、肛温,计算免疫脏器指数。用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术检测大鼠盲肠内容物代谢物,通过正交偏最小二乘法-判别分析,输入“京都基因与基因组百科全书”数据库,筛选出潜在差异代谢物及可能的代谢通路。结果 干预结束后,实验组、对照组、模型组和空白组的平均体质量分别为(216.87±7.85)、(210.96±9.03)、(159.47±5.18)和(293.51±22.98)g,肛温分别为(36.14±0.48)、(35.40±0.64)、(34.50±0.78)和(36.61±0.34)℃,胸腺指数分别为(1.19±0.20)、(1.24±0.25)、(0.47±0.15)和(1.31±0.21)mg·g^(-1),脾指数分别为(1.95±0.33)、(2.18±0.28)、(1.61±0.27)和(2.29±0.24)mg·g^(-1);与模型组相比,实验组和对照组大鼠的上述指标均显著升高(均P<0.01)。筛选出14种炙红芪干预脾气虚大鼠的潜在生物标志物,主要通过氨基酸代谢、核黄素代谢及腺苷酸活化蛋白激酶代谢等途径发挥作用。结论 炙红芪可通过改善脾气虚大鼠盲肠内容物代谢水平,保护肠黏膜屏障,减少肠道炎症反应,从而发挥健脾补气的作用。Objective To find potential biomarkers and analyzing metabolic pathways of the treatment by honey-processed Hedysari Radix,the cecal contents of rats with spleen-Qi deficiency were used as samples for analysis.Methods Sixty male SD rats were randomly divided into blank,model,experimental and control groups.The rats in other groups except the control group were carried out by using the three-factor compound modeling method of bitter-cold diarrhea,excessive exertion and hunger and satiety disorders.Experimental group was given12.60 g·kg^(-1) honey-processed Hedysari Radix;control group was given 0.63 g·kg^(-1) lactobacillus bifidum triplex tabletsa;control and model groups received with equal volume of distilled water for a total of15 days.Measure body weight,anal temperature,immune organ index of rats.Ultra-pressure liquid chromatography-quadrupole-exactive-mass spectrometry technology was used to measure the levels of endogenous metabolites in cecum contents.Orthogonal partial least squares discriminant analysis and database " Kyoto Encyclopedia of Genes and Genomes" were used to identify potential differential metabolites and possible metabolic pathways.Results After the intervention,the average body weight of the experimental,control,model and blank groups was(216.87±7.85),(210.96±9.03),(159.47±5.18) and(293.51±22.98)g;anal temperature was(36.14±0.48),(35.40±0.64),(34.50±0.78) and(36.61±0.34) ℃;the thymus indexes were(1.19±0.20),(1.24±0.25),(0.47±0.15) and(1.31±0.21) mg·g^(-1);the spleen indexes were(1.95±0.33),(2.18±0.28),(1.61±0.27) and(2.29±0.24) mg·g^(-1).Compared with the model gr oup,the above indexes of the experimental group and the control group were significantly increased(all P <0.01).A total of 14potential biomarkers of Honey-processed Hedysari Radix in treating spleen-Qi deficiency syndrome were screened out in this study,which mainly involved amino acid metabolism such as tryptophan and glutamate,riboflavin metabolism and adenosine 5 '-monophosphate-activated protein kinase m

关 键 词：炙红芪 脾气虚证 盲肠内容物 代谢组学 

分 类 号：R28[医药卫生—中药学]