臭灵丹提取物调控TGF-β/BMPs信号通路对股骨粗隆间骨折固定术后感染大鼠的影响  

Effects of Choulingdan extract modulating TGF-β/BMPs signaling pathway on infected rats after fixation of femoral intertrochanteric fracture

作　　者：王佳玮 苏育南 潘德艺 黄晓磊[1] 曾华火 徐诗雄 WANG Jia-wei;SU Yu-nan;PAN De-yi;HUANG Xiao-lei;ZENG Hua-huo;XU Shi-xiong(The Second Affiliated Hospital of Fujian Medical University,Quanzhou,Fujian 36200O,China)

机构地区：[1]福建医科大学附属第二医院急诊科,福建泉州362000

出　　处：《中华医院感染学杂志》2024年第4期542-546,共5页Chinese Journal of Nosocomiology

基　　金：泉州市科技计划基金资助项目(2018Z097)。

摘　　要：目的分析臭灵丹提取物调控转化生长因子-β/骨形成蛋白(TGF-β/BMPs)信号通路对股骨粗隆间骨折固定术后感染大鼠的影响.方法选取SPF级Wistar大鼠44只,10只大鼠作为空白组,剩余34只建立股骨粗隆间骨折固定术后感染模型,共有30只大鼠建模成功,将其分为模型组、药物对照组、臭灵丹提取物组3组,每组10只.药物对照组给予阿莫西林,臭灵丹提取物组给予臭灵丹提取物,空白组、模型组不做处理,干预7 d后观察大鼠变化.结果与空白组相比,模型组、药物对照组、臭灵丹提取物组肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、Ⅰ型胶原羧基端肽β特殊序列(β-CTX)水平上升,IL-10、Ⅰ型原胶原N-端前肽(PⅠNP)、骨碱性磷酸酶(BALP)水平、转化生长因子-β(TGF-β)mRNA、骨形态发生蛋白-2(BMP-2)mRNA表达量及相对表达量下降(P<0.05);与模型组相比,药物对照组、臭灵丹提取物组TNF-α、IL-6、β-CTX水平下降,IL-10、PⅠNP、BALP、TGF-βmRNA、BMP-2 mRNA表达量及相对表达量上升(P<0.05);与药物对照组相比,臭灵丹提取物组TNF-α、IL-6、β-CTX水平下降,IL-10、PⅠNP、BALP水平、TGF-βmRNA、BMP-2 mRNA表达量及相对表达量上升(P<0.05).结论股骨粗隆间骨折固定术后感染大鼠经臭灵丹提取物干预后炎性反应减轻,骨代谢改善,其机制可能与TGF-β/BMPs信号通路激活有关.OBJECTIVE To analyze the effect of Choulingdan extract modulating TGF-β/BMPs signaling pathway on infected rats after fixation of femoral intertrochanteric fracture.METHODS A total of 44 SPF Wistar rats were selected,10 rats were used as the blank group,and the remaining 34 rats were used to establish the infection mod-el after fixation of intertrochanteric fracture of the femur.A total of 30 rats were modeled successfully,which were divided into 3 groups,including model group,drug control group and Kuolingdan extract group,with 10 rats in each group.The drug control group was given amoxicillin,the Choulingdan extract group was given Chouling-dan extract,the blank group and the model group were not treated,and the changes of the rats were observed af-ter intervention for 7 days.RESULTS Compared with the blank group,the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and carboxy-terminal peptideβ(β-CTX)of typeⅠcollagen were increased in model group,drug control group and Choulingtang extract group,while the levels of interleukin-10(1L-10),typeⅠprocollagen N-terminal propeptide(PⅠNP),bone alkaline phosphatase(BALP),transforming growth factor beta(TGF-β)mRNA and Bone morphogenetic protein(BMP-2)mRNA expression and their relative expression levels were de-creased(P<0.05).Compared with model group,the levels of TNF-a,IL-6 andβ-CTX in drug control group and Kuol-ingdan extract group were decreased,while the mRNA expressions and relative expressions of IL-10,PINP,BALP,TGF-βmRNA and BMP-2 were increased(P<0.05).Compared with drug control group,the levels of TNF-α,IL-6 andβ-CTX were decreased,while the mRNA expression levels of IL-10,PⅠNP,BALP,TGF-βmRNA and BMP-2 mR-NA were increased(P<0.05).CONCLUSION The inflammatory response was alleviated and bone metabolism was im-proved in infected rats after fixation of intertrochanteric fracture of femur by intervention with the extract of Choulingdan,and the mechanism might be related to the activation of TGF-β/BMPs signaling pathway.

关 键 词：股骨粗隆间骨折 感染 臭灵丹提取物 转化生长因子-β/骨形成蛋白 

分 类 号：R619.3[医药卫生—外科学]