气溶胶微生物粒子本征荧光计数仪器设计  

Design of intrinsic fluorescence counting instrument for aerosol microbial particles

作　　者：贾纹纹 谭军 张秦[3] 党磊 徐溢 陈李[1,2] JIA Wenwen;TAN Jun;ZHANG Qin;DANG Lei;XU Yi;CHEN Li(Key Laboratory of Optoelectronic Technology and Systems,Ministry of Education,School of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;Key Laboratory of New Micro Nano Devices and System Technology,Chongqing University,Chongqing 400044,China;Aerospace Shenzhou Biotechnology Group Co Ltd Beijing Space Bioengineering Research Center,Beijing 100080,China)

机构地区：[1]重庆大学光电工程学院光电技术与系统教育部重点实验室,重庆400044 [2]重庆大学新型微纳器件与系统技术重点学科实验室,重庆400044 [3]航天神舟生物科技集团有限公司北京市空间生物工程研究中心,北京100080

出　　处：《传感器与微系统》2024年第3期80-83,共4页Transducer and Microsystem Technologies

基　　金：国家重点研发计划资助项目(2021YFC2103300);国家自然科学基金资助项目(61971074);中央高校基金资助项目(2022CDJKYJH031)。

摘　　要：针对气溶胶微生物粒子实时监测的需求,对基于微生物粒子本征荧光和光散射检测方法和装置进行了研究。分析了粒子光散射检测的基本原理,对微生物粒子荧光活性分子以及金黄色葡萄球菌等6种微生物进行了荧光光谱的测试和分析,确定了405nm激发光和双路散射光的整体设计方案。通过对气路和检测腔结构的重要参数仿真,实现了其优化设计,最后,完成了检测装置的构建。初步测试结果表明:该装置实现了对粒子散射光和荧光的同步探测,可以对普通尘埃粒子和微生物粒子进行区分和计数,为进一步开展气溶胶微生物粒子浓度的实时监测仪器研发奠定了基础。Aiming at the demand for real-time monitoring of aerosol microbial particles,the detection methods and devices based on microbial particles intrinsic fluorescence and light scattering are studied.The basic principle of particle light scattering detection is analyzed.Then,the fluorescence spectra of microbial particles flourescent active molecules and Staphylococcus aureus and other six microorganisms are tested and analyzed.The overall design schemes of 405 nm excitation light and two-way scattered light are determined.Through the simulation of the important parameters through the gas path and the detection cavity structure,the optimal design is realized.Finally,the construction of the detection device is completed.The preliminary test results show that the device realizes the synchronous detection of particle scattered light and fluorescence,and can distinguish and count ordinary dust particles and microbial particles,which lays a foundation for further research and development of real-time monitoring instrument for aerosol microbial particle concentration.

关 键 词：生物光学 微生物粒子 实时监测 本征荧光 粒子计数 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] E933.43[自动化与计算机技术—控制科学与工程] Q631[军事—军事装备学]