具有内表面拓扑结构的仿生血管设计与制备  

Design and Fabrication of Biomimetic Blood Vessels with Internal Surface Topological Structure

作　　者：杜搏达 亓剑[1] 张国亮 郑淑贤 Du Boda;Qi Jian;Zhang Guoliang;Zheng Shuxian(Tianjin Key Laboratory of High Speed Cutting and Precision Machining,Tianjin Engineering Research Center for High-End Intelligent CNC Machine Tools,School of Mechanical Engineering,Tianjin University of Technology and Education,300350,China;Tianjin Key Laboratory of Equipment Design and Manufacturing Technology,School of Mechanical Engineering,Tianjin University,Tianjin 300354,China)

机构地区：[1]天津职业技术师范大学机械工程学院天津市高端智能数控机床工程研究中心天津市高速切削与精密加工重点实验室,天津300350 [2]天津大学机械工程学院装备设计与制造技术天津市重点实验室,天津300354

出　　处：《微纳电子技术》2024年第3期126-135,共10页Micronanoelectronic Technology

基　　金：国家自然科学基金项目(52175275);天津市研究生科研创新项目(2022SKYZ131);天津市教委科研计划项目(2021KJ032);天津职业技术师范大学预研项目(KYQD202210)。

摘　　要：针对临床人工血管不具备诱导内皮化的内表面微结构,无法为内皮细胞生长提供合适的力学环境的问题,设计并制备了一种具有内表面拓扑结构的仿生血管(BVST),实现了壁面剪切力的优化分布。基于内皮细胞的形态分布,设计了具有正交、菱形、圆弧三种内表面拓扑结构的仿生血管。通过仿真分析,发现正交与菱形结构的BVST对血液流速影响较小,周期波动相近,且BVST的内表面拓扑结构能减缓细胞流速、增加细胞滞留时间,有利于内皮细胞在进行体外培养时沉积、黏附在BVST内表面。通过对比BVST与无拓扑结构仿生血管的剪切力分布,发现BVST整体剪切力分布更加均匀。分析单个拓扑单元内的剪切力分布,发现菱形拓扑单元内部剪切力分布均匀且连续,剪切力变化较小,有利于内皮细胞的生长。将聚己内酯(PCL)溶于二氯甲烷(DCM)配制质量分数35%的PCL-DCM溶液,采用内径为0.2 mm的针头,在移动速度40 mm/s、电压5.8 kV、针头高度1 mm、挤出气压0.05 MPa的条件下,基于电纺直写技术制备具有拓扑结构的仿生血管内膜;将拓扑结构浸水黏附在纺丝收集柱,基于静电纺丝技术制备仿生血管外膜,获得具有内表面拓扑结构的双层仿生血管。提出的仿生血管设计与制备工艺可为血管内皮化研究提供参考。In response to the problem of clinical artificial blood vessels lacking internal surface microstructure to induce endothelialization and being unable to provide a suitable mechanical environment for endothelial cell growth on the inner surface,a biomimetic vessel with an interior surface topological structure(BVST)was designed and prepared,achieving optimized distribution of wall shear stress.Based on the morphological distribution of endothelial cells,three interior surface topological structures(orthogonal,rhombic and arc-shaped)were designed for the biomimetic vessel.Through simulation analysis,it is found that BVSTs with orthogonal and rhombic structures have minimal impact on blood flow velocity and have similar periodic fluctuations.Additionally,the interior topological structures of BVSTs can slow down cell flow velocity,increase cell retention time,and facilitate the deposition and adhesion of endothelial cells on the interior surface of BVST during in vitro cultivation.By comparing the distribution of shear stress between BVST and biomimetic vessels without topological structures,it is found that the overall distribution of shear stress in BVST is more uniform.Analysis of the distribution of shear stress within individual topological units reveals that the distribution of shear forces within rhombic topological units is uniform and continuous,with minimal variations in shear stress,which is conducive to the growth of endothelial cells.A polycaprolactone-dichloromethane(PCL-DCM)solution with a mass fraction of 35%was prepared by dissolving PCL in DCM,and the biomimetic vessel with internal topological structure was prepared using electrospinning direct writing technology with a needle inner diameter of 0.2 mm,a moving speed of 40 mm/s,a voltage of 5.8 kV,a needle height of 1 mm,and an extrusion pressure of 0.05 MPa.The topological structure was immersed in water then adhered to the spinning collection column,and the outer membrane of the biomimetic vessel was prepared based on electrospinning technology,r

关 键 词：增材制造 电纺直写 仿生血管 剪切力 拓扑结构 内皮细胞 

分 类 号：TH703[机械工程—仪器科学与技术] TH162.1[机械工程—精密仪器及机械]