基于响应面优化大麦籽粒总黄酮提取及抗氧化活性分析  

Extraction method of total flavonoids from barley grains based on response surface methodology and its antioxidant activity

作　　者：杨涛[1] 环慧敏 陈佳[1] 普晓英[1] 杨晓梦[1] 李娥贤[1] 李霞[1] 杨丽娥 曾亚文[1] YANG Tao;HUAN Hui-min;CHEN Jia;PU Xiao-ying;YANG Xiao-meng;LI E-xian;LI Xia;YANG Li-e;ZENG Ya-wen(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China;Pu’er University,Pu’er,Yunnan 665000,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南昆明650205 [2]普洱学院,云南普洱665000

出　　处：《南方农业学报》2023年第10期3029-3036,共8页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(32160453);现代农业产业技术体系建设专项(CARS-05-01A-04)。

摘　　要：【目的】利用响应面法对大麦籽粒总黄酮提取工艺进行优化,并分析其抗氧化活性,为研究和开发大麦籽粒中黄酮类化合物提供科学参考。【方法】通过单因素试验考察不同振荡方法(普通振荡器振荡和超声波振荡)的提取时间、不同提取液种类(甲醇和乙醇)及其浓度和不同料液比对大麦籽粒总黄酮提取量的影响,确定主要影响因素,然后采用响应面法优化大麦籽粒总黄酮提取工艺,并通过1,1-二苯基-2-三硝基苯肼(DPPH)法评价大麦总黄酮的抗氧化活性。【结果】单因素试验筛选出提取液为乙醇,采用超声波提取。建立回归模型:Y=5968.42+1148.42A-80.73B+84.16C+312.09AB-39.94BC-955.41A2-790.56B2-316.62C2(Y为总黄酮提取量,A为乙醇体积分数,B为料液比,C为超声波提取时间);乙醇体积分数对大麦籽粒总黄酮提取量有极显著影响(P<0.01),料液比的影响最小;两两因素间的交互作用对大麦籽粒总黄酮提取量均无显著影响(P>0.05)。优化的超声波提取大麦籽粒总黄酮工艺条件:乙醇体积分数56%、料液比1∶20、超声波提取时间89 min,在此条件下得到大麦籽粒总黄酮提取量为6201.0µg/g。大麦籽粒总黄酮对DPPH自由基具有一定的清除能力,在50~300µg/mL范围内,清除能力与大麦总黄酮浓度有一定的相关性。【结论】通过响应面法优化的大麦籽粒总黄酮提取工艺可有效提高提取效果,缩短提取时间,提取的大麦黄酮具有较强的抗氧化活性。【Objective】To provide scientific reference for the research and development of flavonoids in barley seeds by using response surface methodology to optimize the extraction process of total flavonoids from barley seeds and analy�zing their antioxidant activity.【Method】By conducting a single factor experiment to investigate the extraction time of dif-ferent oscillation methods(ordinary oscillator oscillation and ultrasonic oscillation),the effects of different types of ex-traction solutions(methanol and ethanol)and their concentrations,as well as different solid-liquid ratios on the extraction of total flavonoids from barley seeds,the main influencing factors were determined.Then,the response surface methodo�logy was used to optimize the extraction process of total flavonoids from barley seeds,and 1,1-diphenyl-2-trinitrophenylhy-drazine(DPPH)was used to evaluate the antioxidant activity of barley flavonoids.【Result】The single factor experiment selected ethanol as the extraction solution and used ultrasonic extraction.The established regression model:Y=5968.42+1148.42A-80.73B+84.16C+312.09AB-39.94BC-955.41A2-790.56B2-316.62C2(Y was the total flavonoid extraction amount,A was the volume fraction of ethanol,B was the solid-liquid ratio,and C was the ultrasonic extraction time).Among them,ethanol volume fraction had extremely significant effect(P<0.01),solid-liquid had the least effect.The interac�tion between the two factors had no significant effect on the extraction of total flavonoids from barley grains(P>0.05).The optimized extraction process conditions for total flavonoids from barley seeds were as follows:ethanol volume frac-tion of 56%,solid-liquid ratio of 1∶20,ultrasonic extraction time of 89 min.Under these conditions,a total flavonoid ex-traction amount of 6201.0µg/g from barley seeds was obtained.The antioxidant results indicated that barley flavonoids had certain scavenging ability against DPPH free radicals.In the range of 50-300µg/mL,the scavenging ability of barley flavonoids again

关 键 词：大麦 总黄酮 超声波提取 响应面法 抗氧化活性 

分 类 号：S512.309.9[农业科学—作物学]