Soil bacterial and fungal communities resilience to long-term nitrogen addition in subtropical forests in China  

作　　者：Xinlei Fu Yunze Dai Jun Cui Pengfei Deng Wei Fan Xiaoniu Xu 

机构地区：[1]School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,Anhui,People’s Republic of China [2]Suzhou Polytechnic Institute of Agriculture,Suzhou 215008,Jiangsu,People’s Republic of China [3]College of Life and Environment Sciences,Huangshan University,Huangshan 245041,Anhui,People’s Republic of China

出　　处：《Journal of Forestry Research》2024年第2期95-108,共14页林业研究（英文版）

基　　金：supported by the National Science Foundation of China(No.31770672 and 3137062);the National Basic Research Program of China(No.2010CB950602)。

摘　　要：Atmospheric nitrogen(N)deposition is predicted to increase,especially in the subtropics.However,the responses of soil microorganisms to long-term N addition at the molecular level in N-rich subtropical forests have not been clarified.A long-term nutrient addition experiment was conducted in a subtropical evergreen old-growth forest in China.The four treatments were:control,low N(50 kg N ha^(-1)a^(-1)),high N(100 kg N ha^(-1)a^(-1)),and combined N and phosphorus(P)(100 kg N ha^(-1)a^(-1)+50 kg P ha^(-1)a^(-1)).Metagenomic sequencing characterized diversity and composition of soil microbial communities and used to construct bacterial/fungal co-occurrence networks.Nutrient-treated soils were more acidic and had higher levels of dissolved organic carbon than controls.There were no significant differences in microbial diversity and community composition across treatments.The addition of nutrients increased the abundance of copiotrophic bacteria and potentially beneficial microorganisms(e.g.,Gemmatimonadetes,Chaetomium,and Aureobasidium).Low N addition increased microbiome network connectivity.Three rare fungi were identified as module hubs under nutrient addition,indicating that low abundance fungi were more sensitive to increased nutrients.The results indicate that the overall composition of microbial communities was stable but not static to long-term N addition.Our findings provide new insights that can aid predictions of the response of soil microbial communities to long-term N addition.

关 键 词：Long-term nitrogen addition Old-growth subtropical forest METAGENOMICS Beneficial microorganisms Co-occurrence network 

分 类 号：S714.3[农业科学—林学]