基于Fe_(3)O_(4)@CeO_(2)过氧化物模拟酶的尿酸检测  

Determination of Uric Acid Based on Peroxidase-like Activity of Fe_(3)O_(4)@CeO_(2)

作　　者：韩素平 李晓红 徐宁宁 付正 Han Suping;Li Xiaohong;Xu Ningning;Fu Zheng(Department of Pharmacy,Shandong Medical College,Jinan Shandong 250002,China;Department of Professional Foundation,Jinan Vocational College of Nursing,Jinan Shandong 250102,China;Shandong Hongjitang(Shanghe)Pharmaceutical Group Co.,Ltd.,Jinan Shandong 251600,China)

机构地区：[1]山东医学高等专科学校药学系,山东济南250002 [2]济南护理职业学院专业基础部,山东济南250102 [3]宏济堂制药(商河)有限公司,山东济南251600

出　　处：《山西化工》2024年第2期44-45,64,共3页Shanxi Chemical Industry

基　　金：山东省医药卫生科技发展计划项目(202011001041);山东省中医药科技项目(2021M219,M-2023253)。

摘　　要：两步水热法制备的四氧化三铁@二氧化铈(Fe_(3)O_(4)@CeO_(2))具有过氧化物模拟酶活性,可催化H2O2氧化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色的氧化产物(TMBox)。通过与尿酸酶对尿酸的催化反应进行级联,开发了高灵敏、高特异性的尿酸比色检测新方法。该方法测定尿酸的线性范围为10~100μmol/L,检测限为7.9μmol/L,结果准确可靠。A two-step hydrothermal method was employed to synthesize Fe_(3)O_(4)@CeO_(2)nanocomposites which has peroxidase-like activity.Fe_(3)O_(4)@CeO_(2)can catalyze the oxidation reaction of 3,3',5,5'-tetramethylbenzidine(TMB)by H_(2)O_(2) to form a blue colored product(TMBox).A sensitive and selective colorimetric method for uric acid detection was established by cascading with the oxidation reaction of urieoxidase toward uric acid.The detection limit of uric acid was 7.9μmol/L and the linear detection range was from 10 to 100μmol/L.The test result was accurate and reliable.

关 键 词：过氧化物模拟酶 尿酸 比色检测 

分 类 号：O65[理学—分析化学]