荧光偏振法在布鲁菌病抗体检测中的应用  

Application of fluorescence polarization method in the detection of brucellosis antibodies

作　　者：王月[1] 张悦[2] 谭勤琴 刘英[1] 马青[1] 韦小瑜[1] 王铭 WANG Yue;ZHANG Yue;TAN Qinqin;LIU Ying;MA Qing;WEI Xiaoyu;WANG Ming(Department of Bacterial Disease Laboratory,Guizhou Center for Disease Control and Prevention,Guizhou,Guiyang 550000,China;不详)

机构地区：[1]贵州省疾病预防控制中心细菌性疾病检验科,贵州贵阳550000 [2]贵州医科大学公共卫生与健康学院

出　　处：《医学动物防制》2024年第1期101-104,共4页Journal of Medical Pest Control

基　　金：贵州省传染病人才培养基地建设项目子项目(RCJD2102)。

摘　　要：目的 荧光偏振法应用于人体血清布鲁菌病(简称“布病”)抗体检测,分析其在布病诊断中的应用价值,为布病实验室检测提供科学依据。方法 收集贵州省2021年布病重点监测人群的血清245份,同时应用荧光偏振法(fluorescence polarisation method, FPA)、虎红平板凝集试验(rose bengal test, RBT)、血清凝集试验(serum agglutination test, SAT)和酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)进行检测,分析4种检测方法结果间的一致性、敏感性和特异性。结果 FPA与RBT、SAT、ELISA的Kappa值分别为0.852、0.845、0.778,FPA与传统RBT、SAT的Kappa值均>0.750,即FPA与其他3种诊断方法比较,有很高的一致性。ELISA与RBT、SAT、FPA的Kappa值分别为0.779、0.804、0.778,ELISA与其他3种方法的Kappa值为0.610~0.800,即ELISA与其他3种诊断方法比较,有较好的一致性。与确诊试验SAT比较,RBT的敏感性和特异性均高。FPA、ELISA与SAT比较敏感性和特异性也较高,敏感性均为86.2%,但FPA的特异性(98.4%)高于ELISA的特异性(94.3%)。结论 与SAT进行比较,FPA和ELISA敏感性好、特异性强、一致率高、Kappa值较高;RBT一致性较好、敏感性高、特异性一般、易出现假阳性。通过比较和分析4种布病血清抗体的实验室检测方法,荧光偏振法作为一种新技术,有较高的灵敏度和特异度,对布病的防治有重要意义。Objective To apply fluorescence polarization method to detect human serum brucellosis(referred to as"brucellosis")antibody,analyze its application value in the diagnosis of brucellosis,so as provide scientific basis for laboratory detection of brucellosis.Methods A total of 245 sera from key surveillance of population of brucellosis in Guizhou Province in 2021 were collected and simultaneously used fluorescence polarization method(FPA),rose bengal test(RBT),serum agglutination test(SAT)and enzyme-linked immunosorbent assay(ELISA)for detection.The consistency,sensitivity and specificity between the results of the four methods were analyzed.Results The Kappa values of FPA compared with RBT,SAT and ELISA were 0.852,0.845 and 0.778,respectively.The Kappa values of FPA and traditional RBT and SAT were all more than 0.750.This means that there was a high consistency between FPA and the other three diagnostic methods.The Kappa values of ELISA and RBT,SAT and FPA were 0.779,0.804 and 0.778,respectively,and the Kappa values for ELISA versus and the other three methods were between 0.610 and 0.800,ELISA was consistent with the other three diagnostic methods.The sensitivity and specificity of RBT and confirmed to confirmatory test SAT were higher.The sensitivity and specificity of FPA and ELISA were also higher than that of SAT,with a sensitivity of 86.2%,but the specificity of FPA was 98.4%,higher than that of ELISA(94.3%).Conclusion Compared with SAT,FPA and ELISA have better sensitivity,specificity,consistency and Kappa value,while RBT has better consistency,high sensitivity,general specificity and easy to appear false positive.By comparing and analyzing the four laboratory detection methods of brucellosis serum antibodies,fluorescence polarization method as a new technology has higher sensitivity and specificity,which is of great significance for the prevention and treatment of brucellosis.

关 键 词：布鲁菌病 荧光偏振法 血清凝集试验 酶联免疫吸附试验 虎红平板凝集试验 

分 类 号：R516.7[医药卫生—内科学]