基于TGF-β/Smad3分析沉默TLR2基因对慢性尿路感染大鼠的影响  

Analyzing the effect of silencing TLR2 genes on chronic urinary tract infection in rats based on TGF-β/Smad3

作　　者：郑雪君 李润奇 侯桂平 李润姣 崔利 ZHENG Xuejun;LI Runqi;HOU Guiping;LI Runjiao;CUI Li(Department of Sports Medicine,Zhangjiakou Second Hospital,Zhangjiakou,Hebei 075000,China;不详)

机构地区：[1]张家口市第二医院运动医学科,河北张家口075000 [2]张家口市第二医院医学检验科 [3]张家口市第二医院中药剂科

出　　处：《医学动物防制》2024年第3期275-280,285,共7页Journal of Medical Pest Control

基　　金：河北省中医药管理局中药类科研计划项目(2021442)。

摘　　要：目的 探究沉默TLR2基因对慢性尿路感染模型大鼠作用机制。方法 将40只Sprague Dawley(SD)大鼠分为对照组、模型组、阴性对照(negative control, NC)组和试验组,试验组和NC组大鼠建立慢性尿路感染模型,建模后分别尾静脉注射sh-TLR2和sh-NC3试剂,每周1次。对照组和模型组注射等量生理盐水,连续3周比较大鼠体温。检测大鼠膀胱组织Toll样受体2(toll like receptor 2,TLR2)表达水平,同时检测大鼠膀胱和肾脏的菌落数和大鼠的尿白细胞和淋巴细胞计数,另外检测外周血炎症因子。检测各组大鼠膀胱组织的转化生长因子(transforming growth factor, TGF)-β、SMAD家庭成员3(Smad3)mRNA表达水平以及p-Smad3蛋白表达水平。结果 与对照组相比,模型组、NC组和试验组大鼠的体温显著上升,试验组大鼠的体温显著低于模型组和NC组。与对照组相比,模型组和NC组大鼠的膀胱和肾脏组织菌落数均显著升高,试验组大鼠的上述指标与模型组和NC组相比显著降低(模型组:t=-3.616、-22.972,P=0.002、<0.001;NC组:t=-3.950、23.974,P=0.001、<0.001);与对照组相比,模型组、NC组大鼠的尿白细胞和淋巴细胞计数显著上升,试验组低于模型组和NC组(模型组:t=-1.437、-1.082,P=0.168、0.293;NC组:t=-1.376、-0.712,P=0.186、0.485)。与对照组相比,模型组和NC组大鼠外周血的炎症因子显著上升;与NC组和模型组相比,试验组大鼠外周血的炎症因子显著降低。与对照组相比,模型组和NC组大鼠的TGF-β和Smad3 mRNA表达水平上升,与模型组和NC组相比,试验组大鼠的TGF-β和Smad3 mRNA表达水平显著降低(TGF-β:t=-6.371、-7.500,P<0.001;Smad3:t=-8.132、-7.406,P<0.001;p-Smad3:t=-20.586、-16.956,P<0.001)。与对照组相比,模型组和NC组大鼠的TGF-β、Smad3和p-Smad3的表达水平上升;与模型组和NC组相比,试验组大鼠的TGF-β、Smad3和p-Smad3表达水平显著降低(TGF-β:t=-13.483、-23.813,P<0.001;Smad3:t=-24.575、-12.622,P<0.00Objective To investigate the mechanism of silencing TLR2 genes on chronic urinary tract infection in model rats.Methods Forty Sprague Dawley(SD)rats were divided into control group,model group,negative control(NC)group,and experimental group.The experimental group and NC group were used to establish a chronic urinary tract infection model.After modeling,sh-TLR2 and sh-NC3 reagents were injected into their tail veins once a week.The control group and model group were injected with an equal amount of physiological saline,and the body temperature of the rats was compared for three consecutive weeks.The expression level of toll like receptor 2(TLR2)in rat bladder tissue,the bacterial count in rat bladder and rat kidney,as well as the urine white blood cell count and lymphocyte count were detected.The peripheral blood inflammatory factors were also detected.In addition,the transforming growth factor(TCF)-β,level of SMAD family member 3(Smad3)mRNA expression level and p-Smad3 protein expression were detected.Results Compared with the control group,the body temperature of rats in the model group,the NC group and the experimental group significantly increased,while the body temperature of the experimental group rats was significantly lower than that of the model group and NC group.Compared with the control group,the bacterial count in the bladder and kidney tissues of the model group and NC group rats increased significantly,while the above indicators of the experimental group rats reduced markedly compared to the model group and NC group(model group:t=-3.616,-22.972,P=0.002,<0.001;NC group:t=-3.950,23.974,P=0.001,<0.001);compared with the control group,the urine white blood cell count and lymphocyte count of the model group and NC group rats were noticeably increased,with the experimental group lower than the model group and NC group(model group:t=-1.437,-1.082,P=0.168,0.293;NC group:t=-1.376,-0.712,P=0.186,0.485).Compared with the control group,the inflammatory factors in the peripheral blood of the model group and NC

关 键 词：TLR2 慢性尿路感染 自噬 TGF-Β/SMADS 保护作用 分析 

分 类 号：R691.3[医药卫生—泌尿科学]