青海省乌兰县与同德县鼠疫噬菌体分离及流行病学比较分析  

Comparative analysis of isolation and epidemiology of Yersinia pestis Phage in Wulan County and Tongde County of Qinghai Province

作　　者：李翔[1] 赵海红[1] 徐小青[1] 张琪[1] 李存香[1] 杨建国[1] 徐伟 祁芝珍[1] LI Xiang;ZHAO Haihong;XU Xiaoqing;ZHANG Qi;LI Cunxiang;YANG Jianguo;XU Wei;QI Zhizhen(Qinghai Institute for Endemic Disease Prevention and Control,Xining,Qinghai 811602,China;不详)

机构地区：[1]青海省地方病预防控制所,青海西宁811602 [2]青海省乌兰县疾病预防控制中心

出　　处：《医学动物防制》2024年第2期113-116,121,共5页Journal of Medical Pest Control

基　　金：青海省鼠疫防控及研究重点实验室项目(2021-ZJ-Y15)。

摘　　要：目的 对青海省乌兰县和同德县喜马拉雅旱獭采集到的鼠疫噬菌体进行分离及流行病学分析,掌握喜马拉雅旱獭鼠疫疫情的发展趋势和鼠疫防控预警。方法 利用Luria-Bertani(LB)液体培养基和双层琼脂平板进行纯化,分析噬菌体裂解谱,噬菌体分离率比较采用t检验。结果 乌兰县与同德县100份喜马拉雅旱獭肠道样品分别分离到噬菌体14株和1株,分离率为14.00%和1.00%,两地噬菌体的分离率差异有统计学意义(t=3.583,P=0.001);喜马拉雅旱獭的爪子、肝脏和脾脏样品均未分离到噬菌体。鼠疫噬菌体裂解谱结果显示,乌兰县1株噬菌体分离株仅能裂解18株鼠疫耶尔森菌,裂解率为32.73%,其他13株与同德县的1株噬菌体基本可全部裂解所选的55株鼠疫耶尔森菌,裂解率为89.09%~100.00%。结论 乌兰县喜马拉雅旱獭所采集样品的鼠疫噬菌体分离率较高,符合当地动物间鼠疫疫情高发现状;同德县虽长期处于鼠疫疫情静息状态,但喜马拉雅旱獭所采集的样品分离到鼠疫噬菌体,或可间接指示该地区动物间鼠疫疫情的存在。Objective eTo isolate and epidemiologically analyze the Yersinia pestis Phages collected from Marmota himalayana in Wulan County and Tongde County,Qinghai Province,and to grasp the development trend of Marmota himalayana plague epidemic and the early warning of plague prevention and control.Methods Purification was carried out by using Luria-Bertani(LB)liquid culture medium and double-layer agar plate,and the Phage lysis spectrum was analyzed.The separation rate of Phage was compared by t test.Results Fourteen and one strains of Phage were isolated from 100 Marmota himalayana intestinal samples in Wulan County and Tongde County,respectively,with the separation rate of 14.00%and 1.00%,and the difference of separation rate of Phage between the two places was statistically significant(t=3.583,P<0.001).No Phage was isolated from the paws,liver and spleen samples of the Marmota himalayana.The results of plague Phage lysis spectrum showed that 1 strain of Phage isolated from Wulan County could only lyze 18 strains of Yersinia pestis,with the lysis rate of 32.73%,while the other 13 strains and 1 strain of Phage isolated from Tongde County could lyze 55 strains of selected Yersinia pestis,with the lysis rate of 89.09%to 100.00%.Conclusion The isolation rate of Yersinia pestis Phage of samples collected by Marmota himalayana in Wulan County is higher,which is in line with the high discovery rate of plague epidemics among local animals.Although Tongde County is in a long-term resting state of plague epidemics,the isolation of Yersinia pestis Phage in samples collected by Marmota himalayana may indirectly indicate the existence of plague epidemic among animals in this area.

关 键 词：鼠疫噬菌体 分离纯化 裂解谱 鼠疫疫源地 乌兰县 同德县 流行病学 分析 

分 类 号：R516.8[医药卫生—内科学]