澳洲白羊与呼伦贝尔羊杂交对母羊繁殖性能及妊娠期生殖激素分泌的影响  

Effects of Crossbreeding of Australian White Sheep and Hulun Buir Sheep on Reproductive Performance and Reproductive Hormone Secretion During Gestation Period of Ewes

作　　者：陈炯阳 程艳 伍志武 王标 杨燕燕 杨青[1] 贺志雄[2] 谭支良[2] CHEN Jiongyang;CHENG Yan;WU Zhiwu;WANG Biao;YANG Yanyan;YANG Qing;HE Zhixiong;TAN Zhiliang(College of Veterinary Medicine,Hunan Agricultural University,Changsha410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences/Key Laboratory of Agro-ecological Processes in Subtropical Region/National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production/Hunan Research Center of Livestock and Poultry Sciences/Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process/Institutional Center for Shared Technologies and Facilities,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha410125,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot010031,China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所/亚热带农业生态过程重点实验室/畜禽养殖污染控制与资源化技术国家工程实验室/湖南省畜禽健康养殖工程技术研究中心/动物营养生理与代谢过程湖南省重点实验室/中国科学院亚热带农业生态研究所公共技术中心,湖南长沙410125 [3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2024年第1期42-47,共6页Animal Husbandry and Feed Science

基　　金：中国科学院战略性先导科技专项(XDA26050102);湖南省自然科学基金项目(2022JJ10054);湖南省创新型省份建设专项(2019RS3021)。

摘　　要：[目的]探究澳洲白羊作为父本与呼伦贝尔母羊杂交后,对呼伦贝尔母羊繁殖效率和妊娠期生殖激素分泌的影响。[方法]选取呼伦贝尔羊母羊392只,根据配种公羊品种随机分为两组,即以澳洲白羊(n=7)为种公羊的试验组(n=139)和呼伦贝尔羊(n=13)为种公羊的对照组(n=253);母羊生产后,比较两组母羊的受胎率、流产率、双羔率等繁育性能指标以及羔羊初生重;每组选取8只母羊,采集妊娠中期(妊娠60 d)、妊娠后期(妊娠90 d)、预产期前10 d的血液样品,采用ELISA试剂盒检测血清中促卵泡素(FSH)、促黄体素(LH)、孕酮(P_(4))、雌二醇(E_(2))水平。[结果]与对照组相比,试验组母羊受胎率和实际分娩率显著(P<0.05)降低,流产率显著(P<0.05)升高,双羔率极显著(P<0.01)升高,羔羊成活率无显著(P>0.05)差异。试验组母羊所产羔羊的初生重、单羔羔羊初生重、双羔羔羊初生重与对照组相比无显著(P>0.05)差异。试验组母羊3个妊娠时间血清中FSH、LH、P_(4)和E_(2)的分泌水平与对照组相应妊娠时间相比均无显著(P>0.05)差异。对照组母羊在3个不同妊娠时间下血清中LH、FSH、E_(2)分泌水平均无显著(P>0.05)差异,但预产期前10 d血清中P_(4)分泌水平显著(P<0.05)高于妊娠中期和妊娠后期。试验组母羊妊娠后期血清中E_(2)分泌水平显著(P<0.05)高于妊娠中期和预产期前10 d,妊娠中期血清中E_(2)分泌水平显著(P<0.05)高于预产期前10 d;试验组母羊妊娠后期和预产期前10 d血清中P_(4)分泌水平显著(P<0.05)高于妊娠中期。[结论]澳洲白羊作为父本与呼伦贝尔母羊杂交可极显著提高后代的双羔率,从而提升母羊繁殖效率,说明澳洲白羊作为父本,具备一定改良呼伦贝尔繁殖性能的杂交优势。[Objective]This study aimed to investigate the effects of crossbreeding of Australian White sheep as paternal parents and Hulun Buir sheep as maternal parents on reproductive performance and reproductive hormone secretion during gestation period of ewes.[Method]A total of 392 Hulun Buir ewes were selected and randomly assigned into an experimental group or a control group according to the ram breeds.For the ewes in the experimental group(n=139),Australian White sheep(n=7)were served as the breeding ram,while for those in the control group(n=253),Hulun Buir sheep(n=13)were served as the breeding ram.After delivery,the reproductive performance indicators such as conception rate,miscarriage rate and twinning rate of the ewes in the two groups,as well as the birth weights of the lambs produced by the ewes in the two groups were statistically compared.Eight ewes were selected from each group,and blood samples were collected at mid-gestation period(60 d gestation),late-gestation period(90 d gestation)and 10 d before the expected date of lambing,respectively.The serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),progesterone(P_(4))and estradiol(E_(2))were measured with ELISA kits.[Result]The ewes in the experimental group had significantly(P<0.05)lower conception rate and actual delivery rate,significantly(P<0.05)higher miscarriage rate,and extremely significantly(P<0.01)higher twinning rate compared with those in the control group.No significant(P>0.05)difference in lamb survival rate between the experimental group and the control group was observed.There were no significant(P>0.05)differences in the lamb birth weight,single-lamb birth weight and twin-lamb birth weight between the experimental group and the control group.At each observed time points during gestation period,the serum levels of FSH,LH,P_(4) and E_(2)of the experimental group ewes had no significant(P>0.05)differences with those of the control group ewes.For the ewes in the control group,there were no significant(P>0.05)differences

关 键 词：呼伦贝尔羊 杂交 澳洲白羊 繁殖效率 生殖激素 

分 类 号：S826.32[农业科学—畜牧学]