北部湾珊瑚共附生链格孢菌A.alternata GXIMD 02507次级代谢产物研究  

Study on the secondary metabolites from the Beibu Gulf coralassociated fungus A.alternata GXIMD 02507

作　　者：林妙萍 张艳婷 唐振洲 卢护木 王佳熹 覃育宁 刘永宏 罗小卫 LIN Miaoping;ZHANG Yanting;TANG Zhenzhou;LU Humu;WANG Jiaxi;QIN Yuning;LIU Yonghong;LUO Xiaowei(Institute of Marine Drugs,The First Afiliated Hospital,Guangxi Key Laboratory of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学海洋药物研究院,第一附属医院,广西海洋药物重点实验室,广西南宁530200

出　　处：《中国海洋药物》2024年第1期26-32,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(82260692);广西杰出青年科学基金项目(2023JJG140008);广西中医药大学桂派杏林青年英才项目(2022C038);中药学广西一流学科(桂教科研[2022]1号)资助。

摘　　要：目的对来源于广西涠洲岛鹿角杯形珊瑚(Pocillopora damicornis)共附生真菌Alternaria alternata GXIMD 02507进行物种鉴定及次级代谢产物研究。方法采用ITS序列分析鉴定菌种,利用硅胶柱色谱及半制备高效液相色谱(HPLC)等多种色谱学技术对次级代谢产物进行分离纯化,运用核磁共振(NMR)和圆二色谱(CD)技术并对照文献数据对单体化合物进行结构鉴定。结果从该菌大米发酵产物中分离获得8个以交链孢烯类为主的化合物,分别鉴定为交链孢烯(1)、4’-表交链孢烯(2)、二氢交链孢烯A(3)、格链孢酚(4)、saponaroxin A(5)、zinniol(6)、2-(4-羟苯基)乙酸乙酯(7)和光色素(8)。结论从该菌中共分离4个交链孢烯类化合物,2个苯酚类衍生物,1个三环聚酮和1个光色素,研究发现物质的量浓度为20μmol/L时,化合物1~7对脂多糖(LPS)诱导RAW 264.7巨噬细胞产生NF-κB活性具有显著抑制作用。Objective To identify the species and study the secondary metabolites from a fungus Alternaria alternata GXIMD 02507,which was derived from the coral Pocillopora damicornis collected from the Weizhou island.Methods The species of the strain was identified by ITS sequence analysis.The secondary metabolites were separated and purified by various chromatographic methods exemplified by silica gel column chromatography and semi-high-performance liquid chromatography,and their structures were determined by analysis of NMR and CD data as well as by comparison with literature data.Results A total of nine compounds,predominantly belonging to altenuene deravitives,were obtained from its solid rice fermented products,which were determined as altenuene(1),4"-epialtenuene(2),dihydroaltenuene A(3),alternariol(4),saponaroxin A(5),zinniol(6),2-(4-hydroxyphenyl)ethyl acetate(7),and lumichrome(8),respectively.Conclusion Four altenuene deravitives,two phenolics,one tricyclic polyketide,and one lumichrome were characterized from this fungus.Meanwhile,compounds 1–7 exhibited significant inhibition of lipopolysaccharide(LPS)-induced NF-κB activation in RAW 264.7 macrophages at 20μmol/L.

关 键 词：北部湾 造礁珊瑚 链格孢菌 代谢产物 NF-κB 

分 类 号：R931[医药卫生—生药学]