糖转运蛋白SlSWEET12c负向调控番茄对细菌性叶斑病的抗性  

The Sugar Transporter SlSWEET12c Negatively Regulates Tomato Resistance to Bacterial Leaf Spot Disease

作　　者：邢亚男 程杰 刘欣 吕书文[2] 吴媛媛[2] 姜晶[1] XING Yanan;CHENG Jie;LIU Xin;LÜShuwen;WU Yuanyuan;JIANG Jing(College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China;Institute of Vegetables,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]辽宁省农业科学院蔬菜研究所,沈阳110161

出　　处：《园艺学报》2024年第2期321-334,共14页Acta Horticulturae Sinica

基　　金：辽宁省自然科学基金项目(2022020768-JH1/102-02);国家重点研发计划项目(2022YFF1003002)。

摘　　要：根据番茄表达数据库(http://ted.bti.cornell.edu/cgi-bin/)及实时定量分析发现,糖转运蛋白(sugars will eventually be exported transporters)基因SlSWEET12c在番茄感病品种和抗病品种中表达差异最明显。以番茄(Solanum lycopersicum)栽培品种‘中蔬6号’为试材,获得了稳定遗传的SlSWEET12c沉默(RNAi)和过表达(OE)植株。进一步接种细菌性叶斑病病菌丁香假单胞菌(Pst DC3000)后,通过对叶片中细菌生长量、Fv/Fm变化,可溶性糖和淀粉含量、活性氧积累及防御酶活性和PR蛋白相关基因表达情况的分析,明确SWEET12c在番茄抵御PstDC3000过程中的作用。与野生型相比,RNAiSlSWEET12c植株接种Pst DC3000后叶片中的细菌生长量、病斑数明显减少,对Pst DC3000的抗性增强,而OE-SlSWEET12c的叶片发病更严重,细菌菌落数及褐色病斑数增多。RNAi-SlSWEET12c植株在接菌后蔗糖和淀粉含量明显高于野生型,活性氧积累少于野生型,防御酶(POD、SOD、APX)活性和PR蛋白相关基因(NPR1、PR1、PR2、PR5)表达明显升高,而OE-SlSWEET12c植株蔗糖和淀粉含量低于野生型,活性氧积累高于野生型,防御酶活性和PR蛋白相关基因表达明显低于野生型。以上结果表明SlSWEET12c通过影响叶片中糖分的组成,负向调控番茄对细菌性叶斑病的抗性。According to the tomato expression database(http://ted.bti.cornell.edu/cgi-bin/)and real-time quantitative analysis,the sugar transporter(sugars will eventually be exported transporter)gene SlSWEET12c showed the most obvious expression difference in tomato susceptible and resistant varieties.Using the tomato(Solanum lycopersicum)plant‘Zhongshu 6’as the test material,stable genetic SlSWEET12c silencing(RNAi)and overexpression(OE)plants were obtained.After further inoculation with Pseudomonas syringae(Pst DC3000),the role of SWEET12c in resisting Pst DC3000 in tomato was clarified through the analysis of bacterial growth,Fv/Fm change,soluble sugar and starch content,reactive oxygen species accumulation,defense enzyme activity and PR protein related gene expression in leaves.Compared with the wild type,the number of bacterial colonies and brown spots were significantly reduced in the leaves of RNAi-SlSWEET12c plants vaccinated with Pst DC3000,and the resistance to Pst DC3000 was enhanced,while OE-SlSWEET12c leaves became more severe,and the number of bacterial colonies and brown spots increased.RNAi-SlSWEET12c plants have significantly higher sucrose and starch content than wild type,reactive oxygen accumulation is less than wild type,the activity of defense enzymes(POD,SOD,APX)and PR protein related genes(NPR1,PR1,PR2,PR5)expression,while the sucrose and starch content of OE-SlSWEET12c plants is lower than wild type,reactive oxygen accumulation is higher than wild type,defense enzyme activity and PR protein related gene expression is significantly lower than wild type.The above results indicate that SlSWEET12c negatively regulates tomato resistance to bacterial leaf spot disease by affecting the composition of sugars in leaves.

关 键 词：番茄 糖转运蛋白 细菌性叶斑病 SlSWEET12c 

分 类 号：S641.2[农业科学—蔬菜学]