辣椒CaHMA基因家族的全基因组鉴定和表达分析  

The Whole-genome Identification and Expression Analysis for CaHMA Gene Family of Capsicum annuum L.

作　　者：周政 何冠谛[1] 何叶清 张宇乐 杨名方 娄飞 刘瑶 赵福林 唐越岳 苏敏敏 令狐昌英 王维东 何腾兵[1,2] ZHOU Zheng;HE Guandi;HE Yeqing;ZHANG Yule;YANG Mingfang;LOU Fei;LIU Yao;ZHAO Fulin;TANG Yueyue;SU Minmin;LINGHU Changying;WANG Weidong;HE Tengbing(College of Agriculture,Guizhou University,Guiyang 550025,China;New Rural Development Research Institute,Guizhou University,Guiyang 550025,China;Zunyi Acadamy of Agricultural Sciences,Zunyi Guizhou 563000,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学新农村发展研究院,贵阳550025 [3]遵义市农业科学研究院,贵州遵义563000

出　　处：《种子》2024年第1期1-13,28,共14页Seed

基　　金：国家重点专项部省联动计划课题(2022YFD1901505);国家自然科学基金项目(42267038);贵州省科技支撑计划(黔科合支撑[2019]2846号)。

摘　　要：为了研究辣椒HMA家族基因介导的潜在机制,本研究从辣椒中鉴定出了6个辣椒HMA家族基因,并根据序列比对和系统发育分析将其划分为两个亚家族。对辣椒基因家族进行染色体定位、基因结构和基序组成分析,结果表明,辣椒HMA家族基因在进化过程中相对保守。启动子分析表明,大多数辣椒HMA家族基因含有与非生物胁迫相关的顺式作用元件。转录组数据分析表明,CaHMA2~CaHMA6基因在植物的各组织和各发育时期均有表达,而Zn/Co/Cd/Pb亚群的基因在辣椒各生育期不表达。通过qRT-PCR验证了该基因家族成员在镉和铜胁迫下的表达情况,发现在两种重金属的胁迫下,Cu/Ag亚群的CaHMA5基因表达显著上调,表明CaHMA5在镉和铜胁迫中发挥着重要作用。最后通过酵母镉耐受性试验发现,CaHMA5能够提高酵母的镉耐受性使其恢复生长,暗示CaHMA5具有Cd^(2+)转运功能。本研究为深入解析CaHMA5的功能以及培育辣椒新品种提供了理论依据。To investigate the putative mechanisms mediated by HMA genes in Capsicum annuum L.,and divided them into two subfamilies based on sequence alignment and phylogenetic analysis and six Capsicum annuum L.HMA genes were identified.Chromosome mapping,gene structure analysis,and motif composition assessment of the HMA gene family in Capsicum annuum L.species revealed that these genes exhibit a relatively conserved nature during their evolutionary history.Promoter analysis showed that most of the Capsicum annuum L.HMA genes contained cis-acting elements associated with abiotic stress.Transcriptome data analysis showed that the Cu/Ag subgroup(CaHMA2—CaHMA6)genes were expressed in all tissues and developmental stages of plants,while the genes of the Zn/Co/Cd/Pb subgroup were not expressed in all growth stages of Capsicum annuum L..The expression of the gene family members under cadmium(Cd)and copper stress was verified by qRT-PCR.It was found that the expression of CaHMA5 in the Cu/Ag subgroup was significantly up-regulated under two heavy metal stresses,indicating that CaHMA5 played an important role in cadmium and copper stress.Finally,through the yeast cadmium tolerance test,it was found that CaHMA5 could improve the cadmium tolerance of yeast and restore its growth,suggesting that CaHMA5 had Cd^(2+) transport function.This study provided a theoretical basis for further analysis of the function of CaHMA5 and the cultivation of new Capsicum annuum L.varieties.

关 键 词：遵辣1号 HMA基因家族 表达谱 生物信息学 镉胁迫 

分 类 号：S564.1[农业科学—作物学]