蕨麻响应盐碱胁迫的形态及转录组分析  

Morphological and Transcriptome Analysis on Response of Potentilla anserina to Salt-alkali Stress

作　　者：吕博文 李军乔 闫格 王秀梅 李涛 LÜBowen;LI Junqiao;YAN Ge;WANG Xiumei;LI Tao(College of Ecology,Environment and Resources,Qinghai Minzu University,Xining 810007,China;Key Laboratory of High-Value Utilization of Specialty Economic Plants,Xining 810007,China;Qinghai-Tibetan Plateau Germplasm Resources Researchand Utilisation Laboratory,Xining 810016,China;Qinghai-Tibetan Plateau Industry Research Institute,Xining 810007,China)

机构地区：[1]青海民族大学生态环境与资源学院,西宁810007 [2]青海省特色经济植物高值化利用重点实验室,西宁810007 [3]青藏高原种质资源研究与利用实验室,西宁810016 [4]青藏高原蕨麻产业研究院,西宁810007

出　　处：《种子》2024年第1期14-28,共15页Seed

基　　金：青海省中央引导地方科技发展-科技成果转移转化项目(2023ZY020);2023年青海省大学生创新创业训练计划项目(S202310748017);青海民族大学2023年研究生创新项目(54M2023001)。

摘　　要：为研究不同盐碱胁迫对蕨麻生长情况的影响,结合转录组进行测序分析,采用土培法对蕨麻进行不同梯度的NaCl、NaHCO3胁迫处理,分析其株高、茎粗等形态指标对不同浓度盐碱胁迫的响应差异,结合Illumina高通量测序技术进行转录组测序,初步探究盐碱胁迫对其转录水平的影响。结果表明,盐碱胁迫下,蕨麻对盐的耐受能力强于碱,随着胁迫浓度的增高,蕨麻相关农艺性状呈先增加后降低的趋势,整体呈现出“低促高抑”的现象;MDA含量随胁迫浓度的增大呈先升后降的趋势,说明膜脂过氧化明显,且当胁迫浓度超过一定阈值,抗氧化和渗透调节系统均不能缓解其膜系统损伤;转录组测序共产生82.95 Gb Data,各样品Clean Data均达到8.41 Gb及以上,Q 30碱基百分比在93.17%及以上,通过DEGs分析,3个比较组(ck-vs-NaCl、ck-vs-NaHCO3、NaCl-vs-NaHCO3)分别筛选出1 616个、1 590个和1 770个DEGs;GO富集分析结果表明,盐应答、类黄酮生物合成过程、硝酸还原酶(NADPH)等GO Term被显著富集;KEGG分析表明,苯丙烷生物合成、黄酮类化合物生物合成、MAPK信号途径和植物激素信号转导等通路被显著富集;并且在DEGs中鉴定的ERF、MYB、NAC、WRKY、MYB和bZIP转录因子家族成员最多。蕨麻主要通过提高茎粗和减小小叶面积等方式来适应胁迫环境,整体呈现“低促高抑”的规律,其存在一定阈值。蕨麻通过调节膜成分、氧化还原酶活性、类黄酮生物合成过程、苯丙烷生物合成、MAPK信号途径和植物激素信号转导等生物过程和代谢途径,结合有关转录因子对其所处盐碱环境进行响应。To investigate the effects of different salt-alkali stress on the growth of Potentilla anserina and through transcriptome sequencing analysis,the epigenetic morphological regulation and molecular mechanisms of salt-alkali tolerance in Potentilla anserina were preliminarily explored.Soil cultivation was employed to subject Potentilla anserina to varying concentrations of NaCl and NaHCO 3 stress.The responses of morphological traits,such as plant height and stem thickness,to different levels of salt-alkali stress were analysed.In addition,Illumina high-throughput sequencing technology was utilized for transcriptome sequencing to investigate the impact of salt-alkali stress on the transcriptional levels of Potentilla anserina.Results showed that under salt-alkali stress,Potentilla anserina exhibited higher tolerance to salt than to alkali.As stress concentrations increased,Potentilla anserina's agronomic traits displayed an initial increase followed by a decrease,overall showing a'low promotion,high inhibition'pattern.Malondialdehyde(MDA)content showed an initial increase followed by a decrease with increasing stress concentration,indicating significant membrane lipid peroxidation.When stress concentrations exceeded a certain threshold,both the antioxidant and osmotic regulation systems were unable to alleviate damage to the membrane system.Transcriptome sequencing generated a total of 82.95 Gb of data,with clean data for each sample reaching 8.41 Gb or more,and a Q30 base percentage of 93.17%or higher.Through differential gene expression(DEGs)analysis,1616,1590 and 1770 DEGs in the three comparison groups(ck-vs-NaCl,ck-vs-NaHCO 3,NaCl-vs-NaHCO 3)were identified.GO enrichment analysis of DEGs revealed significant enrichment of terms related to salt response,flavonoid biosynthesis,nitrate reductase(NADPH),and more.KEGG analysis of DEGs indicated significant enrichment of pathways including phenylpropanoid biosynthesis,flavonoid biosynthesis,MAPK signaling,and plant hormone signal transduction.Furthermore,among the id

关 键 词：蕨麻 盐碱胁迫 转录组 转录因子 耐盐碱机制 差异表达基因 

分 类 号：S567.239[农业科学—中草药栽培]