释硅类芽孢杆菌CBP-2的筛选鉴定及对种子萌发的影响  

Screening and Identification of Silicon Solubilizing Paenibacillus xylanilyticus Strain CBP-2 and Its Effect on Seed Germination

作　　者：林勇 习彦花[2] 李俊磊 王丽萍 王素娜 程辉彩[2] LIN Yong;XI Yanhua;LI Junlei;WANG Liping;WANG Su'na;CHENG Huicai(College of Landscape and Ecology,Hebei University of Engineering,Handan Hebei 056038,China;Biology Institute,Hebei Academy of Sciences,Shijiazhuang 050081,China)

机构地区：[1]河北工程大学园林与生态工程学院,河北邯郸056038 [2]河北省科学院生物研究所,石家庄050081

出　　处：《种子》2024年第1期52-58,91,共8页Seed

基　　金：河北省重点研发项目(22322904D,22373803D);河北省科学院高层次人才资助项目(2023G06)。

摘　　要：为发掘和利用高效释硅菌种资源,提升土壤中有效硅含量,本研究利用硅酸盐选择性培养基进行高效释硅菌的分离筛选,对分离得到的释硅菌株进行分类鉴定,测定其解硅能力、抗逆性、溶磷解钾、产铁载体及分泌IAA等促生能力,并通过浸种催芽试验探究其对小白菜种子萌发及幼苗生长的影响。结果表明,从土壤样品中分离筛选得到21株释硅菌株,其中菌株CBP-2在以富硅铁尾矿砂为底物的发酵液中有效硅浓度可达22.46 mg/L,为对照(不接菌)的2.02倍。经形态学、生理生化特征和16S rRNA基因序列分析,将其鉴定为类芽胞杆菌(Paenibacillus xylanilyticus)。释硅菌CBP-2表现出良好的抗逆性,在20~40℃、pH值5~10、NaCl浓度0~7%条件下均可正常生长,最优发酵条件为初始pH值6.0、NaCl浓度4%、30℃培养。菌株CBP-2对磷酸钙和钾长石都有一定的活化利用能力,同时还能产铁载体、分泌IAA。该菌株兼具释硅、溶磷解钾等多种促生功能,同时具有良好的生态适应性,在土壤修复和农业生产中具有潜在的开发应用价值。In order to explore and utilize the resources of high-efficiency silicon releasing bacteria and improve the effective silicon content in the soil,silicate selective culture medium was used for the isolation and screening of high-efficiency silicon releasing bacteria.The isolated silicon releasing bacteria were classified and identified,and their silicon releasing ability,stress resistance,phosphorus and potassium solubilization,iron production carriers,and IAA secretion promoting abilities were measured.The effects of seed soaking and germination inducing experiments on the germination and seedling growth of Chinese cabbage were explored.The results showed that 21 silicon releasing strains were isolated and screened from soil samples,among which strain CBP-2 had an effective silicon concentration of 22.46 mg/L in the fermentation broth using silicon-rich iron tailings as the substrate,which was 2.02 times higher than the non inoculated control.After morphological,physiological and biochemical characteristics,and 16S rRNA gene sequence analysis,it was preliminarily identified as Paenibacilli xylanilicus.Silicon releasing bacterium CBP-2 exhibitted good stress resistance and could grow normally at 20℃-40℃,pH 5-10,and NaCl concentration 0%-7%.The optimal fermentation conditions were initial pH 6.0,NaCl concentration 4%,and cultivation at 30℃.Strain CBP-2 had certain activation and utilization abilities for calcium phosphate and potassium feldspar,while also producing iron carriers and secreting IAA.This strain had multiple growth promoting functions such as silicon release and phosphorus solubilization,as well as good ecological adaptability.It had potential development and application value in soil remediation and agricultural production.

关 键 词：释硅 类芽孢杆菌 筛选 抗逆性 种子萌发 

分 类 号：Q945[生物学—植物学]