利用小孢子培养技术创制红菜薹早熟DH系  

Creation of Early-maturing DH Lines of Brassica campestris var. purpuraria through Microspore Culture Technique

作　　者：赵丽芬 邓英[2] 付文苑 王青青 杨巍 ZHAO Lifen;DENG Ying;FU Wenyuan;WANG Qingqing;YANG Wei(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州省农业科学院园艺研究所,贵阳550006

出　　处：《种子》2024年第1期137-145,共9页Seed

基　　金：贵州省科技计划项目(黔科合支撑[2020]1Y090号);贵州省科技计划项目(黔科合支撑[2022]一般);贵州省科技计划项目(黔科合服企[2022]005);国家自然科学基金项目(31960595);贵阳市蔬菜种质资源研究中心建设项目(筑科合同[2021]5-1号);贵州省蔬菜现代农业产业技术体系项目(GZCYTX2023-01)。

摘　　要：为获得早熟型的双单倍体(DH)植株,加快早熟型红菜薹育种进程,本研究以15个早熟型红菜薹品种为试材,进行小孢子培养,观察小孢子发育过程并对再生植株进行倍性鉴定。结果表明,当花瓣/花药(P/A)长度比值为0.6~0.8时,红菜薹小孢子72%~78%处于单核靠边期;共有6个基因型红菜薹出胚,其中出胚率最大的为gy21-55,平均出胚率为14.3胚/蕾;选取子叶形胚进行植株再生,成苗率在90%以上;利用流式细胞仪对再生植株进行倍性鉴定,平均自然加倍率达76.8%。gy21-40、gy21-55表现出早熟特性,与对照植株相比获得的DH植株可分别提早5 d和8 d抽薹。In order to obtain early maturing double haploid(DH)plants and accelerate the breeding process of early maturing Brassica campestris var.purpuraria.This study used 15 early maturing Brassica campestris var.purpuraria varieties as test materials for microspore culture,observing the development process of microspores and identifying ploidy of regenerated plants.The results indicated that when the petal/anther(P/A)length ratio was between 0.6 and 0.8,approximately 72%to 78%of the microspores of red amaranth were in the uninucleate stage at the periphery;A total of 6 genotypes of red amaranth were able to produce embryos,with gy21-55 exhibiting the highest embryogenesis rate,the average embryogenesis rate was 14.3 embryos per bud;The cotyledonary embryos were selected for plant regeneration,and the plantlet conversion rate exceeded 90%;Flow cytometry was utilized to determine the ploidy level of the regenerated plants,with an average natural doubling rate of 76.8%.Gy21-40 and Gy21-55 exhibited early maturity traits,and the DH plants obtained from them showed a respective advancement of 5 days and 8 days in bolting compared to the control plants.

关 键 词：红菜薹 游离小孢子培养 植株再生 早熟型DH系 

分 类 号：S634.5[农业科学—蔬菜学]