菖蒲郁金汤通过调节抽动-秽语综合征模型大鼠小胶质细胞及神经递质平衡发挥抗抽动作用的实验研究  

Experimental study on the anti-twitching effect of Changpu Yujin Tang by regulating microglia and neurotransmitter balance in a rat model of Tourette syndrome

作　　者：罗文珍 尚菁 史正刚 冯鹏 孙治前 罗凤霞 葛坤祖 杨菊平 董锐霞 LUO Wenzhen;SHANG Jing;SHI Zhenggang;FENG Peng;SUN Zhizian;LUO Fengxia;GE Kunzu;YANG Juping;DONG Ruixia(Clinical College of Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730101

出　　处：《中医儿科杂志》2024年第1期1-8,共8页Journal of Pediatrics of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81960886);甘肃省高等学校创新基金项目(2020A-081);甘肃省自然科学基金项目(20JR10RA324);甘肃省重点研发计划项目(20YF3FA041)。

摘　　要：目的探讨菖蒲郁金汤通过调节小胶质细胞(MG)极化对3,3′-亚氨基二丙腈(IDPN)诱导的抽动-秽语综合征(TS)模型大鼠细胞因子及神经递质的影响。方法选取雄性SD大鼠40只,按照随机数字表法分为空白组、模型组、硫必利组和菖蒲郁金汤组,各10只。使用IDPN腹腔注射制备TS模型,采用运动行为评分表检测造模是否成功。硫必利组给予硫必利混悬液,菖蒲郁金汤组给予菖蒲郁金汤药液,按15 mL/(kg·d)灌胃,空白组和模型组给予等体积生理盐水。造模成功后各组分别取5只大鼠,处死并取材;各组剩余5只大鼠干预28 d后全部处死并取材。观察各组大鼠纹状体组织形态的病理改变、MG的极化分型及纹状体细胞因子[白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)和神经递质多巴胺(DA)、多巴胺受体(DR)、γ-氨基丁酸A型受体(GABAAR)]的水平变化。结果干预前,各组间行为学评分比较,差异均无统计学意义(P>0.05),具有可比性;干预后,硫必利组和菖蒲郁金汤组的刻板行为评分和运动行为评分均较模型组显著降低,差异均有统计学意义(P<0.05),菖蒲郁金汤组的刻板行为评分与硫必利组比较,差异有统计学意义(P<0.05),而2组的运动行为评分比较,差异无统计学意义(P>0.05)。TS大鼠纹状体组织病理切片显示,干预前空白组大鼠纹状体组织排列整齐且紧密、细胞大小适中,神经元核仁较大而圆,形态规则,细胞核与间质轮廓清晰;模型组纹状体组织排列稀疏且紊乱,神经元部分发生变性、坏死,细胞核固缩,胞质肿胀。干预后,硫必利组纹状体神经细胞数量、体积和形态变化与模型组比较无明显区别;菖蒲郁金汤组纹状体神经细胞数量有所增加,胞体变圆,神经元排列紊乱程度及胞质肿胀程度减轻,皱缩坏死减少。干预前,与空白组比较,TS模型组、硫必利组和菖蒲郁金汤组�Objective To investigate the effects of calamus and turmeric root-tuber decoction on cytokines and neurotransmitters in 3,3'-iminodipropionitrile(IDPN)-induced TS rat model by regulating microglia(MG)polari-sation.Methods Forty male SD rats were selected and divided into a blank group,a model group,a thiabendazole group,and a calamus and turmeric root-tuber decoction group,10 each according to the random number table meth-od.The TS model was prepared by intraperitoneal injection using IDPN,and the success of modelling was detected using the motor behaviour scale.The thiabendazole group was given thiabendazole suspension,the Changpu Yujin Tang(CYT)group was given Changpu Yujin Tang medicinal solution by gavage at the amount of 15 mL/(kg·d),and the blank group and model group were given equal volumes of saline.After successful modelling,5 rats were taken from each group,executed and sampled;the remaining 5 rats in each group were all executed and sampled 28 days after the intervention.Pathological changes in the histomorphology of the striatum,polarised typing of MG and changes in the levels of striatal cytokines[interleukin-1β(IL-1β),IL-6,IL-10,tumour necrosis factorα(TNF-α)and the neurotransmitters dopamine(DA),dopamine receptor(DR),γ-aminobutyric acid type A receptor(GABAAR)]were observed in the rats in each group.Results Before the intervention,the comparison of behav-ioural scores between the groups was comparable with no statistically significant difference(P>0.05);after the in-tervention,the stereotyped behavioural scores and motor behavioural scores of the thiabendazole group and the CYT group were significantly reduced compared with those of the model group,and the differences were all statistically significant(P<0.05),and the difference of the stereotyped behavioural scores of the CYT group was statistically significant when compared with that of the thiabendazole group(P<0.05),while the difference between the motor behaviour scores of the two groups was not statistically significant(P>0.05).Histopa

关 键 词：抽动-秽语综合征 菖蒲郁金汤 小胶质细胞极化 细胞因子 神经递质 实验研究 

分 类 号：R285.5[医药卫生—中药学]