基于网络药理学探讨黄芩治疗痤疮活性成分及作用机制  

Active Components and Mechanism of Scutellaria Baicalensis Georgi against Acne Based on Network Pharmacology

作　　者：王庆芬[1] 陈根光[1] 黄丽珊 杨丽娜[1] 田君鹏[1] WANG Qing-fen;CHEN Gen-guang;HUANG Li-shan;YANG Li-na;TIAN Jun-peng(The 909th Hospital of Joint Logistic Support Force/Dongnan Hospital of Xiamen University,Zhangzhou 363000,China)

机构地区：[1]联勤保障部队第909医院/厦门大学附属东南医院,福建漳州363000

出　　处：《解放军药学学报》2024年第1期19-24,共6页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：联勤保障部队第909医院自主科研(面上)项目,No.22MS002。

摘　　要：目的基于网络药理学方法,探索黄芩治疗痤疮的主要活性成分及其分子作用机制。方法运用中药系统药理学分析平台筛选黄芩的主要活性成分及相应的靶蛋白,通过GeneCards、OMIM和TTD数据库筛选出痤疮的相关靶点基因,应用Cytoscape 3.8.2软件构建黄芩作用靶点的网络可视图。运用STRING数据库绘制蛋白质相互作用网络并进行拓扑分析;使用DAVID数据库对成分-疾病的共有靶点基因进行相互作用分析,包括GO功能注释和KEGG通路分析。结果筛选出黄芩活性成分28个,有效作用的核心靶点35个,蛋白质相互作用分析得到PTGS2、PTGS1、PRKACA、AR、NOS2、PIK3CG等6个核心靶点;KEGG富集分析参与的通路有TP53蛋白参与的癌症通路、HTLV-1感染、TNF、PI3K-AKT等信号通路。结论黄芩通过多成分、多靶点和多通路发挥抗痤疮作用,为临床用药提供了新思路。Objective To analyze the active components and potential molecular mechanism of Scutellaria baicalensis Georgi against acne based on network pharmacology approaches.Methods The components and core targets of Scutellaria baicalensis Georgi were screened via the TCMSP database,The target genes related to acne were retrieved from GeneCards,OMIM and TTD databases while the common targets of drugs and related diseases were obtained using Cytoscape 3.8.2 software to construct the target network to screen key active ingredients.Protein interaction analysis was performed through the String platform to construct a PPI network and screen key target genes.GO enrichment analysis and KEGG pathway enrichment analysis were performed on common targets using the David database.Results Twenty-eight compounds of Scutellaria baicalensis Georgi and 35 effective targets were screened out.The PPI network found that PTGS2,PTGS1,PRKACA,AR,NOS2 and PIK3CG were the key targets for Scutellaria baicalensis Georgi.KEGG pathway analysis revealed TP53 pathways in cancer,HTLV-I infection,TNF signaling pathway and PI3K-AKT signaling pathway.Conclusion Multiple components,targets,and pathways are involved in the mechanism of action by which Scutellaria baicalensis Georgi is effective for acne.

关 键 词：黄芩 痤疮 网络药理学 信号通路 

分 类 号：R969[医药卫生—药理学]