水芹总酚酸体外抗HIV-1的作用  

In Vitro Anti HIV-1 Effect of Total Phenolics Acid Extracted from Oenanthe Javanica

作　　者：黄紫晴 于珊 黄正明 马丽英[4] 刘青川 HUANG Zi-qing;YU Shan;HUANG Zheng-ming;MA Li-ying;LIU Qing-chuan(Beijing Weijian Jiye Institute of Biotechnology,Beijing 100039,China;Wuya College of Innovation,Shenyang Pharmaceutical University,Shenyang 110016,China;Fushun Inspection&Detection Certification Center,Fushun 113000,China;National Center for AIDS/STD Control and Prevention,CDC,Beijing 100050,China)

机构地区：[1]北京卫健基业生物技术研究所,北京100039 [2]沈阳药科大学无涯创新学院,辽宁沈阳110016 [3]抚顺市检验检测认证中心,辽宁抚顺113000 [4]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050

出　　处：《解放军药学学报》2024年第1期25-28,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：中国医药教育协会2020重大科学攻关问题和医药技术难题科研课题,No.2020KTZ001。

摘　　要：目的探讨水芹总酚酸体外抑制人类免疫缺陷病毒1型的作用。方法用不同稀释度的水芹总酚酸与TZM-bl细胞共同培养,使用MTT法检测活细胞的数量,观察水芹总酚酸对TZM-bl细胞的毒性作用;用人类免疫缺陷病毒1型实验室适应株SF33、BAL分别感染TZM-bl细胞,基于TZM-bl细胞的荧光素酶检测体系,采用荧光素酶活性检测方法检测水芹总酚酸抗人类免疫缺陷病毒1型的活性。结果水芹总酚酸对TZM-bl细胞表现出较低的细胞毒性,半数细胞毒浓度>1600μg·ml^(-1)。水芹总酚酸对TZM-bl细胞HIV-1 SF33、HIV-1 BAL病毒的半抑制浓度平均值分别为38.93和24.25μg·ml^(-1),治疗指数分别为>41和>66。结论水芹总酚酸在体外具有抑制人类免疫缺陷病毒1型复制活性的作用。Objective To investigate the role of total phenolics acid of Oenanthe Javanica in inhibiting human immunodeficiency virus type 1 in TZM-bl cells.Methods Cultured TZM-bl cells were treated with total phenolics acid extracted from Oenanthe Javanica at different concentrations and the numbers of living cell were calculated using the MTT assay.The anti-human immunodeficiency virus type 1 activity of total phenolics acid extracted from Oenanthe Javanica was assessed using the luciferase detection method.TZM-bl cells were infected with human immunodeficiency virus type 1 laboratory-adapted strains SF33 and BAL respectively and served as the basis for the luciferase detection system.Results The total phenolics acid extracted from Oenanthe Javanica exhibited low toxicity towards TZM-bl cells,with the median cytotoxic concentration exceeding 1600μg·ml^(-1).The average half maximal inhibitory concentrations were determined as 38.93 and 24.25μg·ml^(-1)for total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 SF33 and total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 BAL groups,respectively.In addition,the therapeutic indexes exceeded 41 and 66,respectively.Conclusion These data suggest that total phenolics acid extracted from Oenanthe Javanica can inhibit human immunodeficiency virus type 1 replication in vitro to some extent.

关 键 词：水芹总酚酸 人类免疫缺陷病毒1型 抗病毒活性 

分 类 号：R512.91[医药卫生—内科学] R285.5[医药卫生—临床医学]