核桃炭疽病菌中GH互作蛋白找寻及遗传关系分析  

Analysis on interacting proteins of glycoside hydrolase proteins in pathogens led to walnut anthracnose and their genetic relationship

作　　者：张凯强 韩长志[1,2] ZHANG Kaiqiang;HAN Changzhi(College of Biodiversity Conservation,Southwest Forestry University;The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Kunming,Yunnan 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院 [2]云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《福建农林大学学报（自然科学版）》2024年第2期199-205,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31960314);云南省“兴滇英才支持计划”青年人才专项(YNWR-QNBJ-2020-188);云南省教育厅科学研究基金项目(2023Y0725);云南省研究生导师团队建设项目(2022100)。

摘　　要：【目的】植物病原真菌中的糖苷水解酶(GH)是参与破坏植物细胞壁的重要酶类,在真菌致病过程中发挥着重要作用。了解核桃炭疽病菌中GH蛋白的互作蛋白及其遗传关系,可为深入研究该病菌中GH家族蛋白的协同作用机制提供参考。【方法】基于前期所获得的胶孢炭疽菌Cg1中20个GH蛋白,通过STRING网络在线分析与其相似性较高的两个炭疽菌种——胶孢炭疽菌Cg-14和果生炭疽菌Nara gc5相关蛋白的互作关系;同时,利用生物信息学分析工具对这些蛋白进行了分子对接、亚细胞定位及遗传关系分析。【结果】两个炭疽菌菌株GH蛋白的互作情况极为相似。其中:GH5的互作蛋白数量最多,且GH5、GH6、GH7、GH11之间均存在明显互作关系;GH28亚家族中两个蛋白之间互作但不与其他GH亚家族蛋白互作;与单独GH蛋白存在互作关系的其他类蛋白多参与多糖催化、碳水化合物代谢等过程。不同蛋白之间均存在多个相互作用的氨基酸位点;仅GH72亚家族中的3个蛋白定位于质膜,其余蛋白均定位于细胞外;胶孢炭疽菌Cg1、Cg-14以及果生炭疽菌Nara gc5中GH蛋白明显分为四大类。其中:GH43亚家族中的3个蛋白分属于不同分支,亲缘关系较远;GH5中的3个蛋白有两个属于同一分支,另一个单独在一个分支;而GH7、GH11、GH28亚家族内部的蛋白均属于同一分支。【结论】核桃炭疽病菌中GH蛋白的互作关系主要有3种,即不同GH蛋白之间的互作、同一GH亚家族中不同蛋白之间的互作以及单独的GH蛋白。其中,大部分GH蛋白定位于细胞外,且大多数GH亚家族中不同蛋白之间的亲缘关系较近,仅有小部分亲缘关系较远。【Objective】Glycoside hydrolase(GH),an important enzyme in plant pathogenic fungi,can breach plant cell wall and plays a primary role in the pathogenic process.Understanding the interacting proteins of GH proteins in pathogens of walnut anthracnose and their genetic relationship will provide references for further study on the synergistic mechanism of GH family proteins.【Method】Based on 20 GH proteins identified in Colletotrichum gloeosporioides Cg1 in previous study,their interactions with related proteins in C.gloeosporioides Cg-14 and C.fructicola Nara gc5,2 strains with high similarity with Cg1,were firstly analyzed through online STRING,and then dissected on molecular docking,subcellular localization and genetic relationship by bioinformatics analysis tools.【Result】The interaction networks of GH proteins in the 2 strains were very similar.Among them,GH5 subfamily had the highest number of interacting proteins,and there were obvious interactions among GH5,GH6,GH7 and GH11 subfamilies;2 proteins in GH28 subfamily interacted with each other but not with other GH subfamilies;proteins that interacted with individual GH proteins were mostly involved in polysaccharide catalysis and carbohydrate metabolism pathways.Molecular docking and subcellular localization showed multiple interacting amino acid sites between different proteins,and GH proteins were mostly located outside the cells except for 3 proteins in GH72 subfamily which were located in plasma membrane.GH proteins in Cg1,Cg-14 and Nara gc5 were distinctly divided into 4 branches:3 proteins in GH43 subfamily were far away from each other and belonged to different branches;2 of the 3 GH5 proteins were clustered into a common branch,and the 3rd one was in a single branch;proteins in GH7,GH11 and GH28 subfamilies all belonged to the same branch.【Conclusion】The interaction between GH proteins in C.gloeosporioides mainly includes the interaction between different GH proteins,the interaction between different proteins in the same GH subfamily and th

关 键 词：胶孢炭疽菌 果生炭疽菌 互作蛋白 生物信息学分析 遗传关系 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]