酒瓶椰子叶枯病病原菌鉴定  

Identification of pathogen responsible for leaf blight of Hyophorbe lagenicaulis

作　　者：范绍斌 苏济钰 樊改丽 阮志平[4] 张华峰 胡艳萍 贾悦 梁欣妤 王宗华[1,2] 胡红莉 FAN Shaobin;SU Jiyu;FAN Gaili;RUAN Zhiping;ZHANG Huafeng;HU Yanping;JIA Yue;LIANG Xinyu;WANG Zonghua;HU Hongli(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Geography and Oceanography,Minjiang University,Fuzhou,Fujian 350108,China;Xiamen Administration Center of Afforestation,Xiamen,Fujian 361004,China;Xiamen Botanical Garden,Xiamen,Fujian 361003,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]闽江学院地理与海洋学院,福建福州350108 [3]厦门市绿化中心,福建厦门361004 [4]厦门市园林植物园,福建厦门361003

出　　处：《福建农林大学学报（自然科学版）》2024年第2期206-213,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(U1805232、U1803232、31800008);福建省住房和城乡建设系统2017年第一批科学技术项目(2017-K-90);福建农林大学科技创新基金项目(KFB23031A)。

摘　　要：【目的】近年来,福建省厦门市园林植物园内的酒瓶椰子频发叶枯病,影响了植株的正常生长,降低了酒瓶椰子的观赏价值。为了更好地防控酒瓶椰子叶枯病,对引起该病害的病原菌进行了种类鉴定。【方法】从厦门市园林植物园收集了12份染病的酒瓶椰子叶片样品,通过分离和纯化,得到5个类拟盘多毛孢属真菌菌株。结合其形态特征和基于3个基因(ITS、TUB2和TEF1)的系统发育分析结果将这5个菌株鉴定到种。最后用柯赫氏法则验证所得菌株的致病性。【结果】这5个菌株被鉴定为Neopestalotiopsis cubana、Pestalotiopsis diploclisiae、P.humicola、P.neolitseae和Pseudopestalotiopsis theae。柯赫氏法则验证结果显示,N.cubana、P.diploclisiae、P.humicola和Ps.theae可以引起酒瓶椰子叶片发病,而P.neolitseae不能侵染酒瓶椰子的叶片。【结论】N.cubana、P.diploclisiae、P.humicola和Ps.theae是引起酒瓶椰子叶枯病的病原菌。这是新拟盘多毛孢属(Neopestalotiopsis)和假拟盘多毛孢属(Pseudopestalotiopsis)真菌在酒瓶椰子上的首次报道。其中,N.cubana为我国首次发现。【Objective】The pathogen that frequently led to the leaf blight of bottle palms[Hyophorbe lagenicaulis(L.H.Bailey)H.E.Moore]in Xiamen Botanical Garden of Fujian Province was identified to address the problems of retarded growth and reduced ornamental value of H.lagenicaulis.【Method】Twelve diseased leaf samples of H.lagenicaulis were collected from Xiamen Botanical Garden,and isolation and purification of the strains as well as subsequent morphological analysis and 3-loci(ITS,TUB2 and TEF1)phylogenetic analyses were performed to dissect the strains.【Result】Up to 5 strains were identified as Neopestalotiopsis cubana,Pestalotiopsis diploclisiae,P.humicola,P.neolitseae and Pseudopestalotiopsis theae,respectively.Verification of Koch's postulates showed that N.cubana,P.diploclisiae,P.humicola and Ps.theae can infect the leaves of H.lagenicaulis,while P.neolitseae cannot.【Conclusion】N.cubana,P.diploclisiae,P.humicola and Ps.theae were the pathogens responsible for the leaf blight of H.lagenicaulis.This is the first report of Neopestalotiopsis and Pseudopestalotiopsis species on H.lagenicaulis,and first report of N.cubana in China.

关 键 词：酒瓶椰子 叶枯病 类拟盘多毛孢属真菌 形态学 多基因系统发育分析 

分 类 号：S432.1[农业科学—植物病理学]