正交设计优选酶解辅助——超声提取花榈木叶总黄酮研究  

Optimization of Enzymolysis Assisted Ultrasonic Extraction of Total Flavonoids from leaves of Ormosia henryi by Orthogonal Design

作　　者：蒋亚超 姜文峰 张辉[1] 米承能 谭昕 姚飞虹[1] JIANG Ya-chao;JIANG Wen-feng;ZHANG Hui;MI Cheng-neng;TAN Xin;YAO Fei-hong(Xiangnan University,Hunan Chenzhou 423000;The First People s Hospital of Chenzhou,Hunan Chenzhou 423000,China)

机构地区：[1]湘南学院,湖南郴州423000 [2]郴州市第一人民医院,湖南郴州423000

出　　处：《广州化工》2023年第20期75-78,共4页GuangZhou Chemical Industry

基　　金：郴州市科技计划重点研发计划资助项目(项目编号:zdnk202101);湖南省自然科学基金资助项目(项目编号:2021JJ80074);2021湘南学院校级科学研究资助项目(项目编号:2021XJ34)。

摘　　要：为研究花榈木叶总黄酮提取工艺,以单因素考察实验为基础,并建立以提取时间(A)为10 min、20 min、30 min,乙醇浓度(B)为60%、70%、80%,液料比(C)为1∶20、1∶25、1∶30,提取次数(D)为1、2、3次的四因素三水平正交实验,以期得到花榈木总黄酮酶解辅助-超声提取最佳工艺。研究得出:花榈木总黄酮酶解辅助-超声提取最优方法为:D_(3)B_(3)C_(3)A_(2),即酶解时间20 min,80%乙醇,液料比1∶30,提取3次。本实验优选出的最佳提取方法方便、可靠、提取率高,适合花榈木总黄酮提取。In order to study the extraction process of total flavonoids from the leaves of Ormosia henryi,based on the single factor investigation experiment,and orthogonal experiment of four factors and three levels was established with the extraction time(A)as 10 min,20 min,30 min,ethanol concentration(B)as 60%,70%,80%,liquid material ratio(C)as 1∶20,1∶25,1∶30,and extraction times(D)as 1,2,and 3 times,in order to obtain the best technology of enzymatic hydrolysis assisted ultrasonic extraction of total flavonoids from Ormosia henryi.The results showed that the optimal method of enzymatic hydrolysis assisted ultrasonic extraction of total flavonoids from Ormosia henryi was D_(3)B_(3)C_(3)A_(2):enzymatic hydrolysis time was 20 min,80%ethanol,liquid to material ratio was 1∶30,and extraction was conducted for three times.The best extraction method selected in this experiment was convenient,reliable and has high extraction rate,which was suitable for the extraction of total flavones from Palm.

关 键 词：花榈木叶 总黄酮 正交设计 酶解辅助-超声 

分 类 号：R932[医药卫生—生药学]