一种基于DNA G-四链体结构的汞离子快速检测方法  

A Rapid Detection Method of Mercury Ions Based on DNA G-quadruplex

作　　者：王耀 张梓一 毛明学 高杨 张秀凤 石磊 WANG Yao;ZHANG Zi-yi;MAO Ming-xue;GAO Yang;ZHANG Xiu-feng;SHI Lei(School of Chemical Engineering,North China University of Science and Technology,Hebei Tangshan 063210,China)

机构地区：[1]华北理工大学化学工程学院,河北唐山063210

出　　处：《广州化工》2023年第20期79-82,共4页GuangZhou Chemical Industry

基　　金：2019河北省大学生创新创业训练计划项目(X2019154);河北省自然科学基金青年基金(B2020209079);河北省教育厅学生科研创新项目(JXC2019008)。

摘　　要：随着工业的发展汞污染日渐严重,建立一种快速、准确的Hg^(2+)检测手段变得更加重要。某些富鸟嘌呤(G)的核酸序列能够通过氢键和自身链或者其他序列链上的鸟嘌呤(G)连接折叠成为四链结构,称为G-四链体。Hg^(2+)可以和G-四链体中的胸腺嘧啶T相互作用形成T-Hg^(2+)-T结构,G-四链体也会形成DNA双链结构,这种变化可以影响菁染料的聚集状态,进而引起溶液紫外-可见吸收光谱的变化。基于DNA G-四链体转化菁染料聚集状态开发了一种Hg^(2+)检测手段。With the development of industry,mercury pollution is becoming more and more serious,so it is more important to establish a rapid and accurate Hg^(2+)detection method.Some guanine-rich nucleic acid sequences can be folded into a four-stranded structure by hydrogen bonding to guanine(G)in their own or other sequence.This structure was called G-quadruplexes.Hg^(2+)can interact with thymine T in G-quadruplexes to form T-Hg^(2+)-T structure,and G-quadruplexes can also form DNA double-stranded structure.This change could affect the aggregation state of cyanine dyes,and then cause the change of UV-Vis absorption spectrum.A method was developed for detecting Hg^(2+)based on the fact that DNA G-quadruplexes could change the aggregation state of cyanine dyes.

关 键 词：汞离子 DNA G-四链体 菁染料 快速检测 

分 类 号：O657.3[理学—分析化学]