血清抗体ELISA、TPPA联合检测在梅毒诊断中的应用  

Application of Combined Serum Antibody ELISA and TPPA Test in the Diagnosis of Syphilis

作　　者：刘玲玲 LIU Ling-ling(Clinical Laboratory Department of Shangqiu Municipal Hospital,Shangqiu 476000,Henan Province,China)

机构地区：[1]商丘市立医院检验科,河南商丘476000

出　　处：《罕少疾病杂志》2024年第3期118-119,共2页Journal of Rare and Uncommon Diseases

摘　　要：目的探讨血清抗体酶联免疫吸附试验(ELISA)、明胶颗粒凝集试验(TPPA)联合检测对梅毒的诊断价值。方法选取126例我院2020年8月~2022年8月就诊的疑似梅毒患者,入院后均行血清抗体ELISA、TPPA检查,比较血清抗体ELISA、TPPA对梅毒的诊断结果、诊断效能,并比较其对不同梅毒分期的阳性检出率。结果经ELISA检测显示,阳性37例;TPPA检测显示,阳性38例;ELISA、TPPA联合诊断显示,阳性47例;与血清抗体ELISA、TPPA单独诊断比较,联合诊断灵敏度93.75%、准确率96.03%较高,漏诊率6.25%较低(P<0.05);血清抗体ELISA、TPPA联合诊断对梅毒一期阳性检出率高于单独诊断(P<0.05)。结论血清抗体ELISA、TPPA联合检测能提高梅毒诊断准确性,可用于临床早期筛查、病情分期中,为临床制定相应治疗方案提供依据。Objective To investigate the diagnostic value of serum antibody enzyme-linked immunosorbent assay(ELISA)and gelatin particle agglutination test(TPPA)for syphilis.Methods One hundred and twenty-six patients with suspected syphilis from August 2020 to August 2022 were selected as study subjects,and serum antibody ELISA and TPPA were performed after admission.Results The ELISA test showed 37 positive cases;the TPPA test showed 38 positive cases,and the combined ELISA and TPPA diagnosis showed 47 positive cases;the combined diagnosis had a higher sensitivity of 93.75%,a higher accuracy of 96.03%,and a lower leakage rate of 6.25%(P<0.05);the combined diagnosis of serum antibody ELISA and TPPA had a higher positive detection rate for syphilis stage I than that of the diagnosis alone(P<0.05).Conclusion The combined serum antibody ELISA and TPPA test can improve the diagnostic accuracy of syphilis,and can be used in early clinical screening and disease staging to provide a basis for clinical formulation of the corresponding treatment plan.

关 键 词：酶联免疫吸附 明胶颗粒凝集 梅毒 诊断 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]