EGFR通路在动脉粥样硬化机制中的作用研究  

Study of role of EGFR pathway in mechanism of atherosclerosis

作　　者：涂艳虹 程波 TU Yanhong;CHENG Bo(Department of General Medicine,Wuhan Sixth Hospital,Affiliated Hospital of Jianghan University,Wuhan 430015,China)

机构地区：[1]江汉大学附属医院武汉市第六医院综合科,武汉430015

出　　处：《中国免疫学杂志》2024年第2期278-282,共5页Chinese Journal of Immunology

摘　　要：目的:通过巨噬细胞构建泡沫细胞,探讨EGFR通路在动脉粥样硬化机制中的作用。方法:采用C57BL/6J小鼠巨噬细胞诱导泡沫细胞,油红O染色鉴定模型。qRT-PCR、Western blot检测各靶点干扰效率。qRT-PCR检测IL-6和TNF-α表达。Western blot检测EGFR、p-EGFR、CHOP、ATF4、EIF2α、p-EIF2α和Cyt-C蛋白表达。流式细胞术检测ox-LDL的提取和活性氧的生成。ELISA检测IL-6和TNF-α含量。结果:EGFR抑制剂转染细胞后,EGFR表达下降(P<0.01),其中EGFR sh-RNA3干扰效率最好。EGFR抑制剂(AG1478或EGFR shRNA3)能够显著降低细胞对ox-LDL的提取(P<0.01),显著降低IL-6、TNF-α和活性氧水平(P<0.01),抑制细胞质中Cyt-C表达,显著降低p-EIF2α、Chop和ATF4表达(P<0.01)。结论:EGFR通路可能通过降低炎症和氧化应激减轻动脉粥样硬化。Objective:To investigate role of EGFR pathway in pathogenesis of atherosclerosis by constructing foam cells from macrophages.Methods:Macrophages from C57BL/6J mice were induced into foam cells,and identified by Oil Red O staining.Inter-ference efficiency of each target was detected by qRT-PCR and Western blot.IL-6 and TNF-αexpressions were detected by qRT-PCR.EGFR,p-EGFR,CHOP,ATF4,EIF2α,p-EIF2αand Cyt-C protein expressions were detected by Western blot.Extraction of ox-LDL and formation of reactive oxygen species were detected by flow cytometry.IL-6 and TNF-αcontents were detected by ELISA.Results:EGFR expression inhibitor decreased in turn after transfection of EGFR inhibitor(P<0.01),and interference efficiency of EGFR sh-RNA3 was the best.EGFR inhibitors(AG1478 or EGFR shRNA3)were able to significantly reduce cellular extraction of ox-LDL(P<0.01),significantly reduced levels of IL-6,TNF-αand reactive oxygen species(P<0.01),inhibited expression of Cyt-C in cytoplasm,and decreased expressions of p-EIF2α,Chop and ATF4(P<0.01).Conclusion:EGFR pathway may reduce atheroscle-rosis by reducing inflammation and oxidative stress.

关 键 词：巨噬细胞源性泡沫细胞 动脉粥样硬化 炎症 

分 类 号：R392[医药卫生—免疫学]